暴露纳米氧化锌对雄性小鼠糖、脂代谢及组织学的影响

The influence of nano zinc oxide on glucose,lipid metabolism and hitology in male mice

根据小鼠的体质量,以不同剂量经口连续染毒纳米氧化锌,90d后摘眼球取血,对血脂、血糖、肝脏抗氧化指标进行检测。结果显示:对照组、80mg/kg剂量组小鼠血糖显著高于160mg/kg剂量组(P<0.05)。对照组、80mg/kg剂量组小鼠血糖极显著高于40mg/kg剂量组(P<0.01);40,80 mg/kg剂量组小鼠血脂含量显著高于对照组(P<0.05),160mg/kg剂量组小鼠血脂含量极显著高于对照组(P<0.01);40,160 mg/kg剂量组小鼠血清总胆固醇含量显著高于对照组(P<0.05);40mg/kg剂量组小鼠血清低密度脂蛋白含量显著高于对照组、80mg/kg剂量组(P<0.05),各组间血清高密度脂蛋白含量无显著差异;80,160mg/kg剂量组小鼠肝脏过氧化氢酶活性显著高于对照组、40mg/kg剂量组(P<0.05),80mg/kg剂量组小鼠肝脏过氧化氢酶活性极显著高于40 mg/kg剂量组(P<0.01);40,80mg/kg剂量组小鼠肝脏Cu-Zn SOD活性显著高于对照组(P<0.05);160 mg/kg剂量组小鼠肝脏抑制羟自由基能力显著低于对照组、40mg/kg剂量组及80mg/kg剂量组(P<0.05);随染毒剂量增加,小鼠肝脏MDA含量增加,但各组间含量差异不显著;随染毒剂量增加,小鼠肝脏谷胱甘肽过氧化物酶活性提高;但各组间活性差异不显著。各组间肝脏H2O2含量、总SOD活性无显著性差异。病理学检查发现,大脑细胞存在噬神经现象,脾脏红髓与白髓的界限不清晰;肺泡壁增厚,局部炎性细胞浸润,曲精小管间的间隙增大,生精细胞排列紊乱、脱落。结果表明:经口连续染毒纳米氧化锌90d能影响雄性小鼠肝脏抗氧化系统,导致小鼠糖、脂代谢紊乱,引起高脂血症,纳米氧化锌对雄性小鼠具有神经毒性及生殖毒性。

To detect the effects of nano zinc oxide on mice,the mice were continuously exposed to different doses of nano zinc oxide through intragastric administration. After 90 d,the blood glucose of control group,and 80 mg/kg dose group was significantly higher than 160 mg/kg dose group （P〈0.05）, and 40 mg/kg dose group （P〈0.01）. The blood lipid content of 40 and 80 mg groups was significantly higher than the control group （P〈0.05）. The blood lipid level of 160 mg/kg group was significantly higher than that of control group （P〈0.01）. The serum total cholesterol of 40 and 160 mg groups was significantly higher than that of control group （P〈0.05）. The serum low density lipoprotein content of 40 mg group was significantly higher than that of control and 80 mg groups （P〈0.05）. Serum high density lipoprotein content had no significant differencesamong groups. The liver catalase activity of 80 and 160 mg groups is significantly higher than con trol and 40 mg groups （P〈0.05）. The liver catalase activity of 80 mg group was significantly higher than 40 mg group （P〈0.01）. The liver Cu-Zn SOD activity of 40 and 80 mg groups was significantly higher than control group （P〈0.05）. The hydroxyl free radical inhibiting ability of 160 mg group was significantly lower than the control,40 and 80 mg groups （P〈0.05）. With the increase of infected dose, the MDA content increased, but there was not significant difference a- mong groups. With the increase of infected dose, hepatic glutathione peroxidase activity increased, without significant differences among groups. There was no significant difference of H2 02 content, total SOD activity among groups. Pathological examination revealed neuronophagia of brain cells, the fuzziness boundaries of red pulp and white pulp of spleen. Alveolar wall was thickening, in- flammatory cell infiltration was found in lung, the gap between the seminiferous tubules increased, spermatogenic cell disordered. Results indicate that 90 d exposure to nano zinc oxide can affect the liver antioxidant system, cause lipid metabolism disorder and hyperlipidemia, cause neurotoxicity and reproductive toxicity to male mice.