禽白血病病毒J亚群感染DF-1细胞后错配修复相关基因转录水平变化的研究

Altered transcription levels of the mismatch repair related genes in DF-1 cells infected with the avian leukosis virus subgroup J

为研究禽白血病病毒J亚群(ALV-J)感染DF-1细胞后错配修复相关基因MMR、BRCA1和p53mRNA的转录水平变化,本研究将ALV-J NX0101株接种DF-1细胞,感染7 d后检测ALV-p27抗原,确定病毒感染是否成功。然后提取细胞中总RNA,采用荧光定量PCR以β-actin基因作为内参对MMR(MSH2、MSH3、MLH1和PMS1)、BRCA1和p53基因的mRNA转录情况进行检测。结果表明,与正常对照细胞比较,病毒感染细胞中的MMR(MSH2、MSH3、MLHl和PMSl)和p53基因的mRNA转录水平升高,而BRCA1基因的mRNA转录水平降低。表明在ALV感染宿主细胞后MMR(MSH2、MSH3、MLHl和PMS)、BRCA1和p53参与了宿主细胞DNA损伤的识别及修复过程,这一结果为ALV的致瘤机理的研究提供了新的思路。

The aim of this research is to study the transcription levels of the mismatch repair related genes of MMR （MSH2, MSH3, MLH1 and PMS1）, BRCA1, p53 genes mRNA in DF-1 cells when infected with the avian leukosis virus subgroup J （ALV-J）. DF-1 cells were infected with the ALV-J NX0101 strain and the p27 antigen was detected to confirm the infection. The total RNA was extracted to detect the expression of the mismatch repair related genes by real-time PCR. The transcription levels of MSH2, MSH3, MLH1, PMS1, and p53 mRNA were increased in DF-1 cells after ALV-J infection, but the levels of BRCA1 mRNA were decreased. These results demonstrated that MSH2, MSH3, MLH1, PMS1, BRCA1 and p53 genes response to the recognition and repair of DNA damage. This study provides a new idea for studying the mechanism of tumorigenesis induced by ALV-J.