乙型肝炎病毒核心蛋白区突变与隐匿性乙型肝炎病毒感染的相关性

Mutations in C region of hepatitis B virus may contribute to occult hepatitis B virus infection

目的:探索乙型肝炎病毒核心蛋白区(C区)变异在隐匿性乙型肝炎病毒感染(OBI)中的作用。方法:从2例献血者OBI的样品中扩增得到C区基因构建乙型肝炎病毒C区突变型质粒,检测各突变型质粒在Hu H7细胞中HBs Ag和HBe Ag的水平。Balb/c小鼠高压尾静脉注射突变型质粒检测其血清HBs Ag和HBe Ag的水平,免疫组织化学检测肝组织HBc Ag的表达及分布情况,RT-PCR检测肝组织HBV-RNA的水平。结果:与野生型相比,C区突变型显著性下调HBe Ag和HBc Ag的表达水平,对HBs Ag表达水平和HBV-RNA的水平无显著性作用。结论:OBI患者体内的乙肝病毒C区变异改变HBV病毒的HBe Ag和HBc Ag的表达水平,对OBI的形成具有重要作用。

Objective To investigate the role of mutations in C region that may contribute to occult hepatitis B virus infection. Methods C genes were amplified from two OBI blood donor samples respectively. Plasmids with mutations in C region of hepatitis B virus were constructed by overlapping PCR. HBsAg and HBeAg in Huh7 cells and in the serum of Balb/c mice were detected by CMLA. HBcAg in liver tissue was detected by immunohistochemistry, while HBV-RNA was tested by RT-PCR. Results Mutations in C region significantly reduced the expression level of HBeAg and HBcAg, but had no significant effect on HBsAg and HBV-RNA. Conclusion The mutations in C region affect the expression level of HBeAg and HBcAg, which may play an important role in the occurrence of OBI.