长链非编码RNA CCAT1对子宫内膜癌细胞迁移侵袭及上皮-间质转化的影响

Effect of long non-coding RNA CCAT1 on metastasis,invasion and epithelial-mesenchymal transition of endometrial carcinoma cell

目的:探讨CCAT1对子宫内膜癌细胞迁移侵袭及上皮-间质转化的影响。方法:q PCR检测CCAT1在癌组织中的表达水平,分析其与子宫内膜癌的发生及癌恶性程度的关系;靶向沉默CCAT1,划痕愈合试验和Transwell侵袭试验检测细胞迁移及侵袭能力;q PCR及Western blot检测相关分子的表达水平。结果:CCAT1的表达水平在子宫内膜癌组织中显著增高(P<0.001),且与肿瘤的恶性程度呈正相关;沉默CCAT1后,子宫内膜癌细胞的迁移距离及穿膜数目均显著减少;沉默CCAT1抑制转移相关基因(MMP2和MMP9)及间质标志分子(Snail,Zeb1和Twist1)的表达水平,增强上皮标志分子(E-cadherin和ZO-1)的表达水平。结论:CCAT1在子宫内膜癌中表达上调,且其表达水平与肿瘤的恶性程度呈正相关;沉默表达CCAT1抑制子宫内膜癌细胞的迁移侵袭及上皮-间质转化功能。

Objective To investigate the effect of CCAT1 on migration, invasion and epithelial-mesenchyreal transition （EMT） in endometrial carcinoma （EC） cell. Methods Using quantitative PCR assay, level of CCAT1 was detected in EC tissues to find its association with the initiation and malignancy degree of EC. Wound heal assay and transwell invasion assay were performed to study the effect of CCAT1 on migration and invasion ability of EC cell, while qPCR and western blot assay were utilized to detect the levels of related genes. Results In EC tissues, level of CCAT1 was significantly upregulated （P 〈 0.001） and was positively associated with the malignancy degree of EC. After CCAT1 knockout, shorter migration distance was found, fewer cell passed through the chambers, levels of metastasis-related genes （MMP2 and MMP9） and mesenchymal markers （Snail, Zebl and Twistl） were up-regulated, and epithelial markers （E-cadherin and ZO-1） were down-regulated. Conclusion CCAT1 was up-regulated in EC tissue and its expression level was positively associated with the malignancy degree of EC. CCAT1 knockdown inhibited the metastasis, invasion and epithelial-mesenchymal transition of EC ceil.