摘要
研究白藜芦醇对脑缺血再灌注损伤的作用及机制。利用结扎大脑中动脉的方法制备局灶脑缺血再灌注损伤模型。将实验大鼠随机分为假手术组,脑缺血再灌注损伤组,白藜芦醇组和尼莫地平组,观察白藜芦醇对大鼠肿瘤坏死因子α(TNF-α)、单核细胞趋化因子-1(MCP-1)、白介素-6(IL-6)、Wnt、β-catenin、Bax和BCL-2的表达以及对大鼠神经行为和脑梗死面积的影响。结果显示白藜芦醇可明显改善大鼠神经功能缺陷和减少脑梗死面积,并可上调Wnt、β-catenin、cyclin D1、survivin、和BCL-2的表达,下调促炎症细胞因子TNF-α、MCP-1和IL-6的表达。说明白藜芦醇可激活Wnt/β-catenin信号通路发挥对脑缺血再灌注损伤的保护作用。
The objective of this study was to observe the effect and mechanism of resveratrol on cerebral ischemia reperfusion injury in rats. The Model of cerebral ischemia-reperfusion injury were induced by ligating middle cerebral artery in rats. The rats for study was randomly divided into Sham group,cerebral ischemia reperfusion injury（ IRI）,Resveratrol group（ RSV） and Nimodipine group（ NIM）. The effect of RSV on the cerebral infarct volume and nervous behavior were evaluated. the expression levels of tumor meerosis factor-α（ TNF-α）,Monocyte chemotaxis factor-1（ MCP-1）,interleukin-6（ IL-6）,Wnt,β-catenin,cyclin D1,surviving,Bax and BCL-2 were examined. The result showed that resveratrol could significantly improve the nervous behavior,decrease cerebral infarct volume and attenuate the expression of TNF-α,MCP-1,IL-6 and Bax with increasing the expression of Wnt,β-catenin,cyclin D1,survivin and BCL-2（ P〈 0. 05）. Therefore,it can be concluded that resvtrol can decrease cerebral ischemia-reperfusion injury by activating Wnt/β-catenin signaling pathway.
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2017年第2期212-216,共5页
Natural Product Research and Development
