大蝉虫草发酵液抗真菌活性成分的分离与结构鉴定

Separation and structural identification of antifungal substances in the fermentation broth of Cordyceps cicadae

采用杯碟法对大蝉虫草摇瓶发酵液的乙酸乙酯萃取物进行了抗菌实验,并利用质谱导向的制备高效液相色谱对该乙酸乙酯萃取物中的活性组分进行了跟踪分离得到抗菌活性化合物,根据该化合物的理化性质和波谱数据鉴定其化学结构。结果发现大蝉虫草发酵液的乙酸乙酯萃取物对白色念珠菌有明显的抑制作用,确定白色念珠菌为研究大蝉虫草发酵液抗菌活性的最佳指示菌;从大蝉虫草发酵液中分离得到的抗真菌活性化合物为多壳球菌素。

The broth of Cordyceps cicadae fermented in oscillating manner was extracted with ethyl acetate, and antifunga activity of the extract was tested by Oxford cup method. Active substances from the ethyl acetate extract were purified by high performance liquid chromatography. The chemical structure of the active compound was identified by physio-chemica properties and spectral data. Our experiment showed that the ethyl acetate extract presented a significant inhibiting effect against Canidia albicans, suggesting that Canidia albicans was the best indicator for studying antifungal activity of ethyl acetate extract from fermentation broth of Cordyceps cicadae. The antifungal active substance of the extract was identified as myriocin