三七总皂苷对拟缺血损伤脑微血管内皮细胞PLA2G4A表达及其下游产物的影响

The Effects of PNS on the PLA2G4A And it's Downstream Products in Mimic Ischemic Brain Microvascular Endothelial Cells

目的:观察三七总皂苷(panax notoginseng saponins,PNS)对拟缺血损伤脑微血管内皮细胞胞浆型磷脂酶A2IVA(phospholipase A2 group IVA,PLA2G4A)表达及其功能的影响。方法:培养大鼠原代脑微血管内皮细胞,氧糖剥夺法制备拟缺血模型,用三七总皂苷及PLA2G4A抑制剂进行干预,通过RT-PCR和Western blotting法检测各组PLA2G4A基因及蛋白表达水平,ELISA法检测各组花生四烯酸(arachidonic acid,AA)、20-羟二十烷四烯酸(20-hydroxyeicosatetraenoic acid,20-HETE)、血栓素A2(thromboxanea2,TXA2)/前列环素(prostaglandin I2,PGI2)表达水平。结果:三七总皂苷可显著下调PLA2G4A的基因和蛋白表达,对AA、20-HETE、TXA2/PGI2也有下调作用。结论:三七总皂苷可以下调拟缺血损伤脑微血管内皮细胞PLA2G4A及其下游产物的表达,这可能是三七总皂苷在脑缺血损伤中发挥化瘀通络作用的机制之一。

Objective to evaluate the effects of Panax Notoginseng Saponins （PNS） on the PLA2G4A in Mimic isehemia brain microvascular endothelial cells （BMECs）. Methods： primary rat BMECs were used and mimic cerebral isehemia in vitro was established by oxygen-glucose-deprived （OGD）method. OGD-induced BMECs were treated with PNS and Pyrrophenone（PLA2C,4A inhibitor）respectively. The expression of PLA2G4A in mRNA and protein were detected by RT-PCR and Western blot methods. The expression of AA 20-HETE TXA2/PGI2 were detected by Elisa method. Results ： both mRNA and protein expression of PLA2C,4A in OGD-induced BMECs were down-regulated by PNS. AA 20-HETE TXA2/PGI2 were also decreased in administration group. Conclusion：PNS can down regulate the expression and function of PLA2G4A,which may be the internal mechanism of PNS＇s Anticoagulant effect in cerebral ischemia.