响应面法优化超声辅助提取金银花中绿原酸

Optimization of Ultrasonic-Assisted Extraction of Chlorogenic Acid from Lonicera Japonica by Response Surface Methodology

采用超声辅助乙醇从金银花中提取绿原酸,通过单因素实验考察pH值、料液比、乙醇体积分数和提取时间对绿原酸提取率的影响,采用响应面法优化提取工艺条件。确定最佳工艺条件为:pH值5.2、提取时间21min、乙醇体积分数63%、料液比1∶13.5(g∶mL)。在该优化条件下,绿原酸的提取率达到10.862%。

Chlorogenic acid from Lonicera japonica was extracted using ultrasonic-assisted ethanol. The effects of pH value, solid-liquid ratio,ethanol volume fraction and extraction time on the extraction rate of chlo- rogenic acid was discussed by a single factor experiment, and the extraction conditions were optimized by re- sponse surface methodology. The optimum conditions were as follows： pH value 5.2, extraction time 21 min, ethanol volume fraction 63% ,solid-liquid ratio 1 ： 13.5（g ：mL）. Under the optimum conditions,the extraction rate of chlorogenic acid reached 10. 862%.