蟾酥的化学成分

Chemical Constituents from Bufonis Venenum

目的:研究蟾酥的化学成分,为蟾酥的资源综合利用提供依据。方法:使用正向硅胶,二醇基硅胶,RP-18及LH-20羟丙基葡聚糖凝胶等材料从蟾酥的石油醚、乙酸乙酯、丙酮和甲醇提取部位进行分离纯化,通过理化性质及1D(1H,13C)和2D-NMR(COSY,NOESY,HSQC,HMBC)波谱分析鉴定其化合物的结构。结果:分离纯化得到12个化合物,分别鉴定为isobufalin methyl ester(1),华蟾毒它灵(2),蟾毒灵(3),远华蟾毒精(4),蟾毒它灵(5);蟾毒色胺类为5-hydroxy-Nacetyltryptamine(6),hippophamide(7),其他类为正十五烷酸(8),4,4'-二苯甲烷二氨基甲酸甲酯(9),obtucarbamate A(10),邻苯二甲酸二(2-乙基己基)酯(11),邻苯二甲酸二异辛酯(12)。结论:化合物1为新的天然产物,化合物6~8,10,12为首次从蟾酥中分离得到。

Objective： To study the chemical constituents of Bufonis Venenum. Method： Positive silica gel,glycol silica gel and RP-18,Sephadex LH-20 and other materials were used to isolate and purify the petroleum ether,ethyl acetate,acetone and methanol extracts from Bufonis Venenum,and the structures of the compounds were identified according to their physicochemical properties and 1D（1H,13C） and 2D-NMR（ COSY,NOESY,HSQC,HMBC） spectroscopy analysis. Result： The 12 compounds were identified as： resibufogenins including isobufalin methyl ester（ 1）,cinobufotalin（ 2）,bufalin（ 3）,telocinobufagin（ 4）,bufotalin（ 5）,bufotenidines including 5-hydroxy-N-acetyltryptamine（ 6）,hippophamide（ 7）,and other types including n-pentadecanoic acid（ 8）,4,4＇-diphenylmethane-bis（ methyl） carbamate（ 9）,obtucarbamate A（ 10）,di（ 2-ethylhexyl） phthalate（ 11）,and di（ 6-ethylhexyl） phthalate（ 12）. Conclusion： Compound 1 was a new natural compound and compounds 6-8,10 and 12 were obtained from Bufonic Venenum for the first time.