UPLC测定醉鱼草不同部位中4种黄酮类成分

Determination of Contents of Four Flavonoids in Different Parts of Buddleja lindleyana by UPLC

目的:建立超高效液相色谱(UPLC)同时测定醉鱼草Buddleja lindleyana茎、叶、花、果实中蒙花苷、木犀草素、金合欢-7-O-β-D-葡萄糖苷、金合欢素4种黄酮类成分含量方法,比较在醉鱼草各个部位中4种成分的分布情况。方法:采用Waters Acquity BEH C18色谱柱(2.1 mm×50 mm,1.7μm),流动相乙腈-0.1%甲酸水溶液(梯度洗脱),流速0.15 m L·min-1,检测波长330 nm,柱温30℃,进样体积2μL。结果:蒙花苷、木犀草素、金合欢-7-O-β-D-葡萄糖苷和金合欢素的进样量分别在0.002 0~0.600 3μg(r=0.999 7),0.000 4~0.040 1μg(r=0.999 9),0.000 4~0.039 9μg(r=0.999 9),0.000 2~0.020 0μg(r=0.999 9)和峰面积呈良好的线性关系,仪器精密度及方法重复性均良好,符合定量测定要求。平均回收率分别为100.2%,97.1%,94.3%,92.4%,RSD分别为0.7%,1.1%,2.3%,1.9%。结论:4种黄酮类成分含量在醉鱼草不同部位存在较大差异,其中醉鱼草花中4种黄酮总含量最高,通过比较各不同部位成分的含量差异,为醉鱼草的进一步开发利用提供实验依据。该检测方法操作简单,分离效果好,灵敏度高,可用于醉鱼草不同部位中多个黄酮成分的含量测定。

Objective： To develop a UPLC method for the determination of linarin luteolin,acacia-7-O-β-D-glucopyranoside and acacetin contents in different parts（ stem,leaf,flower and fruit） of Buddleja lindleyana,and compare the distribution of 4 kinds of flavonoids in different parts of B. lindleyana. Method： The optimal conditions of separation and detection were achieved on an Acquity BEH C18column（ 2. 1 mm × 50 mm,1. 7 μm）,with acetonitrile（ A）- 0. 1% formic acid solution（ B） as the mobile phase for gradient elution,at a flow rate of 0. 15 m L·min^-1,with a detection wavelength of 330 nm. The column temperature was 30 ℃ and sample volume was 2 μL. Result： Linarin,luteolin,acacia-7-O-β-D-glucopyranosideand acacetin showed a good linearity in the range of 0. 002 0-0. 600 3（ r = 0. 999 7）,0. 000 4-0. 040 1（ r = 0. 999 9）,0. 000 4-0. 039 9（ r =0. 999 9） and 0. 000 2-0. 020 0 μg（ r = 0. 999 9） respectively. Their average recoveries were 100. 2%,97. 1%,94. 3% and 92. 4%. Conclusion： The method was accurate and simple with high repeatability and stability,and can be used for the determination of multiple flavonoids in different parts of B. lindleyana.