热敏灸对过敏性鼻炎大鼠血清IgE、IL-4含量的影响

Effects of heat-sensitive moxibustion on serum IgE and IL-4 content in rat with allergic rhinitis

目的探讨热敏灸对过敏性鼻炎大鼠血清中IgE、IL-4含量的影响。方法 Wistar雄性大鼠50只,随机分为5组:正常组、模型组、盐酸西替利嗪组、艾灸组和热敏灸组,每组10只。采用卵白蛋白全身致敏与局部攻击相结合的方法复制过敏性鼻炎大鼠模型,观察各组间症状积分的差异,采用ELISA法定量检测各组大鼠血清IgE、IL-4的含量。结果模型组的症状积分与正常组比较差异有统计学意义(P<0.05),各治疗组症状积分明显低于模型组(P<0.05),盐酸西替利嗪组、热敏灸组及艾灸组间比较,盐酸西替利嗪组与热敏灸组比较差异无统计学意义(P>0.05),盐酸西替利嗪组、热敏灸组优于艾灸组(P<0.05);模型组血清中IgE、IL-4的含量明显升高,与正常组比较差异有统计学意义(P<0.05);热敏灸组血清中IgE、IL-4含量明显降低,与模型组比较差异有统计学意义(P<0.05)。结论热敏灸可降低过敏性鼻炎大鼠血清中IgE、IL-4含量,减轻鼻黏膜变应性炎症。

Objective To explore the effect of heat-sensitive moxibustion on serum IgE and IL-4 levels in rats with allergic rhinitis. Methods Fifty Wistar male rats were randondy divided into 5 groups with 10 rats in each group, i.e. normal group, model group, Cetirizine group, moxibustion group and heat- sensitive moxibustion group. The rat model of allergic rhinitis was established by egg-albumin systemic sensitization combined with local attacks. The symptoms in different groups were observed and the scores were calculated. The serum IgE and IL-4 levels were determined by ELISA. Results Compared with the normal group the symptom scores of the model group were significantly high （P 〈 0.05）. The symptom scores of the treatment groups were significantly lower than those of the model group （P 〈 0.05）. There were no significant differences in the symptom scores between the heat-sensitive moxibustion group and the Cetirizine group （P 〉 0.05）. The Cetirizine group and the heat-sensitive moxibustion group were better than the moxibustion group （P 〈 0.05）. Compared with the normal group, the levels of IgE and IL-4 in the model group were significantly increased （P 〈 0.05）; however, the heat-sensitive moxibustion evidently decreased the levels of IgE and IL-4 （P 〈 0.05）. Conclusions Heat-sensitive moxibustion can reduce the serum content of IgE and IL-4 in AR rat, and reduce allergic inflammation of nasal mucosa.