RNAi技术干扰TGF-β/Smads表达对卵巢癌细胞株的影响作用

Effect of RNAi Technique on Ovarian Cancer Cell Lines by Interfering with the Expression of Smads/TGF-β

目的研究RNAi技术干扰TGF-β/Smads表达对卵巢癌细胞株的影响作用。方法将卵巢细胞SKOV3细胞体外培养,随机分为对照组、空白载体组和siRNA干扰组。采用免疫组化法比较三组的TGFRβⅠ、TGFRβⅡ、Smad2、Smad3、Smad4、Smad7表达水平以及细胞生长速度、凋亡率。结果 siRNA干扰组的TGFRβⅠ、Smad7表达水平显著高于对照组和空白载体组(P<0.05),而TGFRβⅡ、Smad2、Smad3、Smad4表达水平低于对照组和空白载体组(P<0.05)。siRNA干扰组接种24h、48 h和72 h时的吸光度显著低于对照组和空白载体组(P<0.05)。siRNA干扰组的细胞凋亡率显著高于对照组和空白载体组(P<0.05)。结论 RNAi技术可干扰TGF-β/Smads表达,对卵巢癌细胞株的生长具有抑制作用。

Objective To study the effect of RNAi technique on ovarian cancer cell lines by interfering with the expression of TGF-fl/Smads. Methods Ovarian SKOV3 cells were cultured in vitro and randomly divided into control group, empty vector group and siRNA interference group. The expression levels of TGFRβI , TGFRβⅡ Smad2, Smad3, Smad4 and Smad7, and cell growth rate and apoptosis rate were compared among three groups by immunohistochemistry. Results The expression levels of TGFRβI and Smad7 of siRNA interference group were significantly higher than those of control group and empty vector group （P 〈0.05）, but the expression levels of TGFRβⅡ, Smad2, Smad3 and Smad4 were lower than those of control group and empty vector group （P〈0.05）. The absorbance at 24 h, 48 h and 72 h in siRNA interference group were significantly lower than those in control group and empty vector group （P 〈0.05）. The apoptosis rate of siRNA interference group was significantly higher than those of control group and empty vector group （P 〈0.05）. Conclusions RNAi technique can interfere with the expression of TGF-β/Smads, so as to inhibit the growth of ovarian cancer cell lines.