塑化剂邻苯二甲酸二丁酯对秀丽隐杆线虫生理活性影响研究

Study on the effects of dibutyl phthalate on physiological activity in Caenorhabditis elegans

目的:探究邻苯二甲酸二丁酯(DBP)对秀丽隐杆线虫的寿命、生殖毒性和神经毒性的影响。方法:将L2期秀丽隐杆线虫暴露于DBP 32.5、131、522 mg/L 3个浓度组、DMSO溶剂对照组和空白对照组中,测定线虫寿命、生殖能力、移动能力和吞咽频率的变化。结果:DBP暴露导致秀丽隐杆线虫的寿命缩短,生殖能力下降,移动能力减慢,吞咽频率降低,且存在剂量依赖关系。结论:DBP暴露缩短线虫寿命,造成生殖毒性和神经毒性影响。

Objective：To study the influence of DBP on lifespan, reproductive toxicity and neurotoxicity in Caenorhabditis elegans. Methods： L2 C.elegans were expoused to the DBP concentration of 32.5,131,522 mg/L. Then nematode longevity, reproductive capacity, mobility and frequency of swallowing were measured. Results：The life span was shorten, reproductive, frequency of swallowing and mobile ability were reduced in Caenorhabditis elegans due to DBP expose in a concentration- dependent manner.Conclusion：DBP induce reproductive toxicity and neurotoxicity in Caenorhabditis elegans, at the same time, it can shorten the lifespan of C.elegans.