摘要
本文采用响应面方法对羊栖菜多糖提取工艺进行了优化,通过超滤膜(MW分别为100000、50000、10000和5000 u)将羊栖菜多糖分进行分离,并对不同分子量段羊栖菜多糖的抗氧化活性进行了分析。在微波功率为385 W时,微波辅助水提法提取羊栖菜多糖的较优工艺条件为:液料比34 m L/g,微波时间8 min,水提温度95℃,提取时间2.1 h,在此条件下,羊栖菜多糖得率为11.51%±0.12%;不同分子量段羊栖菜多糖均具有较强的抗氧化能力,对羟自由基、超氧阴离子、DPPH自由基均有一定程度上的清除作用,SFPSⅤ(MW≤5000 u)自由基清除能力优于SFPSⅠ、SFPSⅡ、SFPSⅢ和SFPSⅣ。在一定范围内,羊栖菜多糖对羟自由基、DPPH自由基清除率超过50%,自由基清除率均随多糖浓度的增加而增强。研究将为羊栖菜多糖的高效利用提供理论指导。
The extraction process of Sargassum fusiforme polysaccharide was optimized by response surface methodology. The polysaccharide fraction of Sargassum fusiforme was separated by ultrafihration membrane ( MW = 100000,50000,10000 and 5000 u), of which the antioxidant activities were studied.When microwave power was 385 W, the microwave-assisted extraction of polysaccharide from Sargassum fusiforme was as follows : the ratio of liquid to material was 34 mL/g, microwave time was 8 min, extraction temperature was 95 ℃, extraction time was 2.1 h, and the yield of polysaccharide was 11.51% ± 0.12%. Under the experiment, the polysaccharide of Sargassum fusiforme had strong antioxidant ability in scavenging hydroxyl radical, superoxide anion and DPPH" free radical, and the ability of SFPS V ( MW ≤ 5000 u ) in scavenging free radical was stronger than SFPS Ⅰ, SFPS Ⅱ, SFPS Ⅲ and SFPS Ⅳ. Radical scavenging rate of hydroxyl radical, DPPH. free radical were more than 50%. Free radical scavenging rate increased with the increasing of polysaccharide concentration. This study will provide theoretical guidance for efficient utilization of Sargassum fusiforme polysaccharide.
作者
刘洪超
应苗苗
周雨暪
杨靖亚
汪之和
施文正
LIU Hong-chao YING Miao-miao ZHOU Yu-men YANG Jing-ya WANG Zhi-he SHI Wen-zheng(College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China Wenzhou Vocational College of Science and Technology ,Wenzhou 325000, China)
出处
《食品工业科技》
CAS
CSCD
北大核心
2017年第6期245-249,255,共6页
Science and Technology of Food Industry
基金
浙江省科技厅科技计划项目(2014C25039)
上海市科委工程中心建设项目(11DZ2280300)
上海市高校知识服务平台项目(ZF1206)
关键词
羊栖菜多糖
微波辅助提取
响应面法
超滤膜
体外抗氧化
Sargassum fusiforme polyccharides (SFPS)
microwave assisted extraction
response surface method
uhrafiltration
antioxdation in vitro