寡雄蛋白(Oligandrin)处理对马铃薯块茎干腐病及苯丙烷代谢的影响

Effects of oligandrin treatment on dry rot and phenylpropanoid pathway metabolism of potato tubers

本实验以"新大坪"马铃薯(Solanum tuberosum)为试材,通过体内实验,研究了寡雄蛋白(oligandrin)对马铃薯块茎干腐病的控制效果;同时从生化和分子水平上分析揭示了寡雄蛋白处理对马铃薯块茎苯丙烷代谢的调控机理。结果表明:56、24、18、12、6μg/m L寡雄蛋白处理对马铃薯块茎干腐病的扩展都有一定的控制效果,其中24μg/m L寡雄蛋白处理效果最佳,处理后块茎病斑直径为对照的58.33%;进一步研究表明寡雄蛋白处理能提高与马铃薯块茎组织苯丙烷代谢相关的苯丙氨酸解氨酶(PAL)、肉桂酸脱氢酶(CAD)、肉桂酸羟化酶(C4H)和4-香豆酰-辅酶A连接酶(4CL)的活性及相关基因的表达;同时抗性物质总酚、类黄酮及木质素含量也因寡雄蛋白处理而提高,在处理后第3 d类黄酮含量出现高峰,高于对照果实54%。可见寡雄蛋白可通过增强组织的苯丙烷代谢而提高马铃薯块茎的抗病性。

Effects of oligandrin on dry rot development of potato tubers （ cv. Xindaping） were evaluated through in vivo tests, regulation mechanism of oligandrin treatment on phenylpropanoid pathway metabolism of potato tubers was also revealed at biochemical and molecular level.The results showed that the dry rot development of potato tuber was effectively controlled with 56,24,18,12 and 6 μg/mL oligandrin treatments in potato tubers, and treatment with 24 μg/mL oligandrin showed the best effect as lesion diameter of potato tubers after this treatment with oligandrin was only 58.33% of the control. Further studies showed that oligandrin enhanced related enzymes activities of phenylpropanoid pathway metabolism including PAL, CAD, C4H, 4CL and the corresponding gene expression of potato tuber. At the same time, antifungal substances such as total phenols, flavonoids and lignin content increased with oligandrin treatment,the content of flavonoids was 54% higher than the control 3 days after treatment.The findings suggested that oligandrin possibly increased disease resistance of potato tubers by enhancing phenylpropanoid pathway metabolism.