基于中药药效物质“敲出/敲入”辨识模式筛选植物化感物质的思考

A consideration on screening of allelochemicals by using "knock-out/in" of targeting ingredients based on identification model of medicinal effect

该文阐述了植物化感作用的概念、特征及其与其连作障碍危害形成间的关系,并结合课题组前期的研究成果,指出精准鉴别化感物质并验证其生态功能是破解植物连作障碍问题的关键。同时,针对化感物质分离鉴定中的难题,借鉴中药药效物质的研究方法,运用目标成分"敲出/敲入"的中药药效物质辨识与质量控制模式,提出了分离鉴定植物化感物质(群)的新思路和新方法。旨在为深化植物化感作用研究和消减中药材生产中连作障碍问题提供理论依据和技术支撑。

This paper clarified in detail the definition,characteristics of allelopathy and its association with consecutive monoculture problem. Most of studies have indicated that it is critical to parse the formation mechanisms of consecutive monoculture problem that identification of allelochemicals and verification of its function. Here,we proposed a new approach to separate and identify the allelochemical group precisely and effectively,in which the ＂ knock-out / in＂ methods of targeting ingredients in the model of medicinal effect identification and quality control were applied. This method will contribute to deep understanding plant allelopathy,and provide theoretical basis and technical support for alleviating consecutive monoculture problems simultaneously.