高效液相色谱法同时测定白芷中欧前胡素和异欧前胡素的含量

Simultaneous determination of imperatorin and isoimperatorin in Radix angelicae by HPLC

目的:建立高效液相色谱(HPLC)法定量检测白芷饮片中欧前胡素和异欧前胡素的含量。方法:色谱柱:Agilent HCC_(18)柱(4.6 mm×150 mm,5μm),以甲醇-水(65∶35)为流动相,柱温25℃,流速1.0mL·min^(-1),检测波长为254 nm。结果:欧前胡素在0.045 2~0.723 2 mg·mL^(-1)范围内线性关系良好(r=0.999 3),异欧前胡素在0.023 8~0.380 8 mg·mL^(-1)范围内线性关系良好(r=0.999 7)。精密度及加样回收率良好。结论:本方法灵敏度高、准确、重复性好,可用于白芷的质量控制。

OBJECTIVE To establish a method for quantitative detection of imperatorin and isoimperatorin in Radix angelicae.METHODS The HPLC system consisted of the Agilent HC-C18（4.6 mm×150 mm,5μm）column,the mobile phase consisted of methanol and water,the flow rate was 1.0mL·min-1,and the column temperature was 25 ℃.UV detector was set at254 nm.RESULTS The linear response range was 0.045 2–0.723 2 mg·mL-1（r=0.999 3）for imperatorin and 0.023 8-0.380 8 mg·mL-1（r=0.999 7）for isoimperatorin.The method showed good precision and average recovery.CONCLUSIONThe method is highly sensitive,accurate and repeatable,and can be used for quality control of Radix angelicae.