刮痧对腰椎间盘突出症模型大鼠背根神经节炎性细胞因子和血清疼痛物质的影响

Effects of Gua Sha therapy on inflammatory factors of DRG and serum pain substances in a lumbar disc herniation rat model

目的观察刮痧对腰椎间盘突出症(LDH)模型大鼠背根神经节(DRG)中炎性细胞因子和血清疼痛物质的影响,探讨刮痧治疗LDH的作用机制。方法 30只雄性SD大鼠随机分为假手术组、模型组、刮痧组。采用自体髓核移植法建立LDH大鼠模型。刮痧组于造模后第5天开始刮痧治疗,假手术组及模型组不予干预措施。干预三个疗程后处死取材。应用免疫组化法检测大鼠背根神经节中炎性细胞因子磷脂酶A_2(PLA_2)和前列腺素E_2(PGE_2)的含量。应用酶联免疫法(ELISA)检测大鼠血清中疼痛物质P物质(SP)和神经肽Y(NPY)的含量。结果与假手术组比较,模型组背根神经节中PLA_2、PGE_2及血清SP、NPY含量均明显升高,差异有统计学意义(P<0.01);与模型组比较,刮痧组背根神经节中PLA_2、PGE_2及血清SP、NPY含量均明显降低,差异有统计学意义(P<0.01)。结论刮痧可有效抑制LDH模型大鼠背根神经节中炎症因子PLA_2、PGE_2和血清疼痛物质SP、NPY的表达,发挥抗炎和神经调节的作用。

Objective To observe the influences of Gua Sha therapy on inflammatory factors of dorsal root ganglion（ DRG） and serum pain substances in a lumbar disc herniation rat model and to explore the therapeutic mechanism of Gua Sha therapy on lumbar disc herniation（ LDH）. Methods A total of 30 male Sprague-Dawley rats were divided into 3 groups randomly： sham operation group,model group and Gua Sha group. The rat model of LDH was established by using autologous nucleus pulpous. Gua Sha group were given Gua Sha therapy on the 5th day after operation. Model group were given no intervention. After three courses of treatment. the ipsilateral L5 DRGs were removed and used for measuring the expression of PLA2 and PGE2 using immune-histochemistry. The serum was collected for measuring the expression of SP and NPY by enzyme linked immunosorbent assay（ ELISA）. Results Compared with the sham operation group,the model group showed significantly increased in expression of PLA2 and PGE2 in DRGs,SP and NPY in the serum（ P 〈0. 01）. Compared with the model group,the Gua Sha group showed significantly decreased in expression of PLA2 and PGE2 in DRGs,SP and NPY in the serum（ P〈 0. 01）. Conclusion Gua Sha therapy can inhibit the expression of PLA2 and PGE2 in DRGs,SP and NPY in the serum of lumbar disc herniation rat model to reach the role of anti-inflammatory and neuromodulation.