马甲子叶中两个新化合物

Two New Compounds from the Leaves of Paliurus ramosissimus

研究马甲子Paliurus ramosissimus(Lour.)Poir叶子乙醇提取物的化学成分.采用硅胶、高效液相制备色谱进行分离纯化得到6个化合物,根据理化性质及NMR、HR-ESIMS等波谱技术鉴定上述化合物的结构分别为2α-O-顺式对羟基肉桂酰基-3α-羟基-27-O-反式对羟基肉桂酰基白桦脂酸(1)、2α-O-反式对羟基肉桂酰基-3α-羟基-27-O-顺式对羟基肉桂酰基白桦脂酸(2)、messagenic acid A(3)、messagenic acid B(4)、白桦脂醛(5)、β-谷甾醇(6).其中化合物1和2为新化合物,命名为马甲子素A和马甲子素B,属于白桦脂酸型五环三萜类化合物;化合物3~6首次从该植物中发现.采用四甲基偶氮唑蓝(MTT)法对化合物1~3进行体外抗肿瘤活性测试,实验结果表明化合物1~3具有显著的体外肿瘤细胞生长抑制活性.

To study chemical constituents in the ethanol extract of Paliurus ramosissimus （Lour.） Poir, two new compounds along with four known compounds were isolated and purified by repeated gel column chromatographies and high performance preparative liquid chromatography. On the basis of spectroscopic methods （HR-ESIMS, 1D-and 2D-NMR）, their structures were identified as 2α-（cis-p-hydroxy-cinnamyloxy）-3α-hydroxy-27-（trans-p-hydroxy-cinnamyloxy）betulinic acid （1）, 2α-（trans-p-hydroxy-cinnamyloxy）-3α-hydroxy-27-（cis-p-hydroxy-cinnamyloxy）betulinic acid （2）, messagenic acid A （3）, mes-sagenic acid B （4）, betulinaldehyde （5） and β-sitosterol （6）. Among them, compounds 1 and 2 were new compounds named as paliurusene A and paliurusene B, which belong to triterpenoids, compounds 3~6 were isolated from this plant for the first time. The anti-tumor activity of compounds 1~3 was evaluated by methyl thiazolyl tetrazolium （MTT） assays. The result showed that compounds 1~3 presented obvious cytotoxicity in vitro against the cancer cell lines.