镉胁迫对赤子爱胜蚓(Eisenia fetida)氧化应激反应影响

Effect of oxidative stress reaction for Eisenia fetida with exposure in Cd

蚯蚓受重金属胁迫时,体内立即产生氧化应激反应,确定胁迫过程氧化应激主要指标对污染监测尤为重要。将胁迫试验分短期和长期试验两组,前者试验周期为10 d,每天取1条蚯蚓;后者试验周期为30 d,每10 d取1条蚯蚓。每组均设定Cd^(2+)浓度梯度50、100、125、250和500 mg·kg^(-1)重金属胁迫溶液,选择蚯蚓环带后尾部测定体内SOD、POD、GPX、GST、CAT、VE、MDA和ACh E。结果表明,短期试验组,反映蚯蚓体内氧化应激主要指标为CAT、SOD和POD。在长期试验中,反映蚯蚓体内氧化应激主要指标为GPX和MDA。

Earthworms are widely used in all kinds of pollutants as sensitive bio-indicator organisms because of their immediately oxidative stress response under the stress of heavy metal. However, there are a large number of indices associated with the oxidative stress response. Finding out the key monitoring indices in the stress process becomes a practical demand of the pollution monitoring and warning process. The paper studied two groups, the short-term test and the long-term test. The former one lasted 10 d, taking out an earthworm everyday. The latter test lasted 30 d, taking out an earthworm every 10 d. The Cd2~ concentration was set at 50, 100, 125, 250 and 500 mg ~ kg1. Post-clitellum segments of earthworms were chosen to determine SOD, POD, GPX, GST, CAT, VE, MDA and ACHE. The results showed that the main bio-indicators associating with oxidative stress reaction in short-term group were： CAT, SOD and POD. While with the long-term test, the main bio-indicators associated with oxidative stress reaction were GPX and MDA.