烟台地区MTHFR基因C677T位点多态性与原因不明复发性自然流产的相关性研究

Study on the Relationship Between the Polymorphism of MTHFR Gene C677T Locus and Unexplained Recurrent Spontaneous Abortion in Yantai

目的探讨亚甲基四氢叶酸还原酶(MTHFR)基因C677T位点多态性与原因不明复发性流产(URSA)的关系。方法采用聚合酶链式反应-限制性片段长度多态性方法(PCR-RFLP),检测烟台地区64例URSA女性和169例正常女性的MTHFR基因C677T位点的多态性。结果 URSA患者的MTHFR基因C677T位点TT型、CC型基因与对照组比较,差异具有统计学意义(P<0.05);TT基因型的个体发生URSA的危险度在不断的增高(OR=8.463,95%CI:2.060~30.475)。结论烟台地区MTHFR基因677位点突变与URSA相关。

Objective To investigate the relationship between methylenetetrahydrofolate reductase（MTHFR） gene C677T polymorphism and unexplained recurrent spontaneous abortion（URSA）. Methods Polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP） was used to detect polymorphism of C677T locus in 64 cases of URSA women and 169 cases of normal women MTHFR gene in Yantai. Results The MTHFR gene C677T locus TT type and CC genotype in URSA patients were statistically significant（P〈0.05）. The risk of URSA in individuals with TT genotype was increasing（OR = 8.463, 95% CI： 2.060 ~ 30.475）. Conclusion 677 mutation of MTHFR gene is associated with URSA in Yantai.