自发性糖尿病长爪沙鼠Rxra在6种组织中的表达水平分析

Analysis of Rxra expression in 6 tissues from spontaneous diabetic Mongolian gerbils

目的分析糖尿病长爪沙鼠Rxra基因在6种组织中的表达水平,以期探索长爪沙鼠糖尿病发生的分子机制。方法选取糖尿病长爪沙鼠和正常沙鼠各6只,分别采集动物的骨骼肌、肝脏、脂肪、肾脏、心脏和脑组织,用Real-time PCR和Western blotting检测Rxra基因在各组织中mRNA和蛋白表达水平。结果 Real-time PCR的结果表明,Rxra基因的mRNA水平在糖尿病组动物骨骼肌和脂肪中显著降低,在肝脏中无差异,而在肾脏、心脏和脑组织中则有高表达趋势。Western blotting结果显示,糖尿病组RXRA的蛋白水平在骨骼肌组织中显著降低,在脂肪组织几乎检测不到,在其他各组织表达情况有所不同,但没有统计学差异。结论 Rxra在糖尿病长爪沙鼠骨骼肌和脂肪组织中低表达,在其他4种组织中表达水平无差异,说明Rxra对长爪沙鼠糖尿病的影响主要发生在骨骼肌和脂肪组织中。

Objective To analyze the Rxra expression level in 6 tissues of diabetic gerbils and explore the molecular mechanism of diabetes in Mongolian gerbils. Methods We collected 6 tissues including skeletal muscle,liver,adipose tissue,kidney,heart and brain from 6 diabetic gerbils and 6 control animals,respectively. Rxra mRNA and protein expression level in the 6 tissues were separately detected using real-time PCR and western blotting. Results The real-time PCR showed that compared with control animal,mRNA expression of Rxra in skeletal muscle and adipose tissue was significantly decreased in the diabetic group. However,there were no significant differences in the liver between the two groups. The relative expression level in diabetic group exhibited uptrend compared with the control in kidney,heart and brain. According to the results of western blotting,RXRA protein in skeletal muscle was significantly decreased in the diabetes group. RXRA protein was almost undetectable in adipose tissue. Varying expression was observed in other tissues,but there was no significant difference. Conclusions Lower expression of Rxra is observed in the skeletal muscle and adipose tissue of diabetic gerbils. There is no significant difference in other four tissues between the two groups. The results illustrate that the effect of Rxra mainly occurres in skeletal muscle and adipose tissue in this diabetic model.