蜘蛛香中绿原酸及总酚酸的含量测定

Determination of Chlorogenic Acid and Total Phenolic Acid Content in Valeriana jatamansi

试验建立了蜘蛛香(Valeriana jatamansi Jones)中绿原酸和总酚酸的含量测定方法。利用高效液相色谱法(HPLC)测定蜘蛛香中绿原酸的含量,色谱柱为Dikma C_(18)(4.6 mm×250 mm,5μm),流动相为乙腈-0.4%磷酸水溶液(12∶88),流速1.0 mL/min,检测波长327 nm,柱温30℃;以绿原酸为对照品,采用紫外-可见分光光度法(UV)在327 nm波长处测定蜘蛛香中总酚酸的含量。结果表明,绿原酸在2~20μg/mL范围内浓度与峰面积线性关系良好,r=0.999 6,加样回收率为97.95%,RSD为1.37%;总酚酸在5~20μg/mL范围内浓度与吸光度线性关系良好,r=0.999 8,加样回收率为100.31%,RSD为1.34%。该方法简便、快速、准确、重复性好,可用于蜘蛛香中绿原酸和总酚酸的含量测定。

To establish a method for determining chlorogenic acid and total phenolic acid in Valeriana jatamansi Jones.Chlorogenic acid content in V.jatamansi was determined by high performance liquid chromatography,as the Dikma ODS（4.6mm×250 mm,5 μm） column was adopted,the mobile phase was acetonitrile-0.4% phosphoric acid（12∶88） at the flow rate of1.0 mL/min,the detection wavelength was 327 nm,and the column temperature was 30 ℃.Total phenolic acid content in V.jatamansi was determined by ultraviolet-visible spectrophotometry spectrophotometer with chlorogenic acid as standard reference at the detection wavelength of 327 nm.Chlorogenic acid showed good linearity in the range of 2 to 20 μg/mL（r=0.999 6）;the recovery was 97.95%（RSD=1.37%）.Total phenolic acid had good linear relationship within the range of 5 to 20 μg/mL（r=0.999 8）;the recovery was 100.31%（RSD=1.34%）.The method is simple,rapid,accurate,reproducible and could be used for measuring the chlorogenic acid and total phenolic acid content in V.jatamansi.