Effects of Cumulus Cells on in vitro Fertilization of Bovine

卵丘细胞对牛体外受精效果的影响（英文）

[Objective] The aim was to explore the effect of cumulus cells on the in vitro fertilization of in vitro matured bovine oocytes. [Method] The in vitro matured oocytes were divided into three groups of cumulus cells removal, partial removal and no removal. [Result] In the co-culture with cumulus cells, the oocytes of the removal group had higher cleavage rate and blastocyst rate （74.4%±4.1, 53.7%±5.1） than those of the no removal group （72.7%±5.1, 52.4%±3.5）, but the difference was not significant （P〉0.05）, while both groups had better performances than the re- moval group （39.6%±4.5, 18.8%±4.6） with the difference reaching the significant level （P〈0.05）. All the three groups showed significant difference with the control. The combination of cumulus cells and melatonin achieved the best effects as the cleavage rate and blastocyst rate of the partial removal group （79.8%±3.7, 56.5%±5.1） were better than those of the no removal group （78.2%±2.6, 55.8%±4.6）, and the difference was not significant, while both group had better performances than the removal group （48.3%±5.5, 22.7%±4.3） and the control group with the differences reaching the significant level （P〈0.05）. [Conclusion] The study provided technical support for the production of dairy cows and beef cattle.

[目的]探索卵丘细胞对牛体外成熟卵母细胞体外受精效果的影响。[方法]试验采用体外成熟后卵母细胞脱除卵丘细胞组、部分脱除组及不脱除组。[结果]与卵丘细胞共培养时,部分脱除组的卵裂率及囊胚率(74.4%±4.1、53.7%±5.1)好于不脱除组(72.7%±5.1、52.4%±3.5),差异不显著(P>0.05)、两者都好于全部脱除组(39.6%±4.5、18.8%±4.6),差异显著(P<0.05),且都显著优于对照组。卵丘细胞与褪黑素两者联合使用时,效果最佳。部分脱除组的卵裂率及囊胚率(79.8%±3.7、56.5%±5.1)好于不脱除组(78.2%±2.6、55.8%±4.6),差异不显著,两者都好于完全脱除组(48.3%±5.5、22.7%±4.3)及对照组,且差异显著(P<0.05)。[结论]该研究对奶牛和肉牛的生产提供了技术支持。