吡唑醚菌酯与戊唑醇复配对葡萄炭疽病菌增效活性与田间防效（英文）

Synergism and Field Efficacy of Mixed Preparations of Pyraclostrobin and Tebuconazole to Glomerella cingulata

[目的]为筛选防治葡萄炭疽病的高效低毒新型复配药剂,降低有效用药量,提高防治效果,防止与延缓抗药性产生。[方法]本研究采用菌丝生长速率法测定了吡唑醚菌酯、戊唑醇及其复配制剂对葡萄炭疽病菌的毒力,并通过田间试验评价了其对葡萄炭疽病的防治效果。[结果]吡唑醚菌酯与戊唑醇及5:1、3:1、1:1、1:3、1:5复配组合对葡萄炭疽病菌的EC50(半最大效应浓度)值分别为1.038 8μg/ml、0.3583μg/ml、0.612 9μg/ml、0.530 1μg/ml、0.2326μg/ml、0.232 8μg/ml和0.329 6μg/ml;5种复配组合对葡萄炭疽病菌的增效系数(SR)分别为1.29、1.33、2.29、1.84、1.22,其中以1:1复配的组合增效作用最大。田间防效调查结果表明,25%吡唑醚菌酯·戊唑醇悬浮剂药剂1 000倍液、2 000倍液、3 000倍液以及430 g/L戊唑醇悬浮剂5 000倍液、250 g/L吡唑醚菌酯乳油2 000倍液对葡萄炭疽病防治效果分别为91.54%、90.80%、82.88%、76.43%、74.10%。[结论 ]因此,吡唑醚菌酯和戊唑醇复配防治葡萄炭疽病增效明显,其中以质量比1:1混合后对葡萄炭疽病菌增效作用最为显著,果穗套袋前采用25%吡唑醚菌酯·戊唑醇悬浮剂1 000~2 000倍液浸果防治葡萄炭疽病害防效均达90%以上。

[Objective] This study was conducted to screen high-efficient low-toxicity new compound preparation for control of Glomerella cingulata. [Method] The toxicity of pyraclostrobin, tebuconazole and their mixed preparations to Glomerella cingulata was determined by mycelial growth rate method, and their control effects on Glomerella cingulata were evaluated by field efficacy test. [Result] The mixed preparations of pyraclostrobin and tebuconazole at mass ratios of 5：1, 3：1, 1：1, 1：3 and 1：5 exhibited ECso values to mycelial growth of Glomerella cingulata of 0.612 9, 0.530 1, 0.232 6, 0.232 8 and 0.329 6 iJg/ml, respectively; and the 5 preparations exhibit- ed synergistic coefficients （SRs） to Glomerella cingulata of 1.29, 1.33, 2.29, 1.84 and 1.22, respectively, and among them, the preparation with mass ratio of 1：1 showed the highest synergism. The field efficacy test showed that the field control effects of 1 000 times, 2 000 times and 3 000 times dilutions of 25% pyraclostrobin-tebuconazole suspension, 5 000 times dilution of 430 g/L tebuconazole suspension and 2 000 times dilution of 250 g/L pyraclostrobin EC were 91.54%, 90.80%, 82.88%, 76.43% and 74.10%, respectively. [Conclusions] Therefore, the mixed preparations of pyraclostrobin and tebuconazole showed remarkable synergism on control of Glomerella cingulata, and preparation with the mixing ratio of 1：1 exhibited the most significant synergism on Glomerella cingulata. For soaking treatment to fruit clusters with 1 000-2 000 times dilution of 25% pyraclostrobin .tebuconazole suspension, the field efficacy to Glomerella cingulata was over 90%.