Maspin基因在不同转移习性的人鼻咽癌细胞株中表达水平的研究

Expression of Maspin gene in human nasopharyngeal carcinoma cell line with different metastatic behavior

目的:在mRNA和蛋白水平检测5种不同转移习性的人鼻咽癌细胞株中Maspin基因表达的差异,探讨Maspin基因与已知转移习性人鼻咽癌细胞株之间的相关性,为鼻咽癌发生转移的可能机制及临床预测转移风险提供理论基础。方法:体外培养SUNE-1-5-8F、SUNE-1、CNE-2Z、CNE-1、SUNE-1-6-10B 5株不同转移习性的人鼻咽癌细胞株。用RT-PCR及Western blot 2种方法在mRNA和蛋白水平检测5种人鼻咽癌细胞株中Maspin基因表达情况。结果:Maspin基因在SUNE-1-5-8F、SUNE-1、CNE-1及SUNE-1-6-10B细胞株均有不同程度表达,差异无统计学意义(P>0.05),在CNE-2Z细胞株中不表达,且在mRNA和蛋白水平Maspin基因表达具有一致性;Maspin基因在不同转移习性人鼻咽癌细胞株中mRNA和蛋白表达量总体存在差异(P<0.05),但表达量不随鼻咽癌细胞株转移习性增高而降低(P>0.05)。结论:Maspin基因在不同转移习性的人鼻咽癌细胞株中mRNA和蛋白表达量不同;Maspin基因可能与人鼻咽癌细胞株的转移潜能无相关性。

Objective： To explore the differences of the expression levels of Maspin mRNA and protein in 5 kinds of human nasopharyngeal carcinoma cell lines with different metastatic behavior,and investigate its correlation for providing the theory basis in studying the metastasis mechanism of nasopharynx cancer and predicting clinical risk. Methods： The SUNE-1-5-8F,SUNE-1,CNE-2Z,CNE-1 and SUNE-1-6-10 B human nasopharyngeal carcinoma cell lines with different metastatic behavior were cultivated in vitro. The expression levels of Maspin mRNA and protein in 5 kinds of human nasopharyngeal carcinoma cell lines were detected using RT-PCR and Western blot. Results： The differences of the expressions of Maspin gene in SUNE-1-5-8F,SUNE-1,CNE-1 and SUNE-1-6-10 B cell lines were not statistically significant（ P〉0. 05）,and the Maspin expression in CNE-2Z cell line could not be detected. The expressions of Maspin mRNA and protein were consistent. The total expression contents of Maspin mRNA and protein in human nasopharyngeal carcinoma cell lines with different metastatic behavior were different（ P〈0. 05）,and the expression of which did not change with the increasing of metastatic behavior of cancer cells. Conclusions： The mRNA and protein expressions of Maspin gene in human nasopharyngeal carcinoma cell lines with different metastatic behavior are different,and the Maspin expression maybe be no-correlation with the metastatic potential of nasopharynx cancer cells.