摘要
为紫苏属植物的种群结构、遗传多样性和进化分类系统研究及种质资源的保护与开发利用提供依据,笔者采用ISSR标记对表型鉴选的10份具有典型植物学性状、来源于不同生态区域的紫苏属植物种系分类进行研究。结果表明:采用ISSR引物进行扩增共获得多态性条带266个,平均多态性比率为56.74%。10份材料间遗传距离为0.690 4-0.975 6,遗传一致度为0.024 7-0.370 4。10份紫苏种质资源可分为2大类群,每个类群包括2个变种。
For studying on population structure, genetic diversity and evolution classification system of PerilZa plants and provide references for protection and exploitation of the Peritla germpIasm resources, 10 Perilla germplasm resource materials possessing various phenotypic characters from different areas were selected to done PCR amplification by using ISSR marker primers. Results. A total of 266 polymorphic bands were finally acquired. The average polymorphic rate was 56. 74%. The variation range of genetic distance from 10 materials was 0. 690 4-0. 975 6, and variation range of genetic coherence was 0. 024 7-0. 370 4. The Perilla materials were classified into two groups, two variants included in each group.
出处
《贵州农业科学》
CAS
2016年第12期10-14,共5页
Guizhou Agricultural Sciences
基金
国家自然科学基金项目"紫苏属植物进化与种群结构研究以及贵州紫苏资源遗传多样性评价"(31360067)
贵州省科学技术基金项目"贵州紫苏属植物SSR标记开发与资源鉴选"[黔科合J字(2014)2020]
贵州省农业科学院专项资金项目"紫苏资源品质研究中近红外光谱分析模型的创建和应用"[黔科合农科院院专项(2011)017]
贵州省科技厅省农科院联合基金项目"转录组及脂肪酸代谢共表达分析揭示紫苏α-亚麻酸合成及调控机理"[黔科合LH字(2015)7062]