基于ISSR标记的10份紫苏种质资源的遗传多样性评价

Genetic Diversity Assessment of 10 Perilla Germplasm Resources based on ISSR Marker

为紫苏属植物的种群结构、遗传多样性和进化分类系统研究及种质资源的保护与开发利用提供依据,笔者采用ISSR标记对表型鉴选的10份具有典型植物学性状、来源于不同生态区域的紫苏属植物种系分类进行研究。结果表明：采用ISSR引物进行扩增共获得多态性条带266个,平均多态性比率为56.74%。10份材料间遗传距离为0.690 4-0.975 6,遗传一致度为0.024 7-0.370 4。10份紫苏种质资源可分为2大类群,每个类群包括2个变种。

For studying on population structure, genetic diversity and evolution classification system of PerilZa plants and provide references for protection and exploitation of the Peritla germpIasm resources, 10 Perilla germplasm resource materials possessing various phenotypic characters from different areas were selected to done PCR amplification by using ISSR marker primers. Results. A total of 266 polymorphic bands were finally acquired. The average polymorphic rate was 56. 74%. The variation range of genetic distance from 10 materials was 0. 690 4-0. 975 6, and variation range of genetic coherence was 0. 024 7-0. 370 4. The Perilla materials were classified into two groups, two variants included in each group.