基于萘酰亚胺骨架的双光子荧光探针构建及应用

Synthesis and application of two-photon fluorescent probes based on naphthalimide

荧光成像技术作为一种可以在无损条件下可视化探究生命活动的手段,近年来得到了广泛的关注。双光子荧光显微镜因拥有背景荧光低,光致损伤小,样品穿透深度大,成像观测时间长等其它成像方法无法比拟的优势已经成为了荧光成像不可或缺的工具。双光子荧光探针作为成像过程中荧光信号的承担者在双光子成像过程中起着至关重要的作用,在目前已报道的众多双光子成像的材料中,基于有机小分子构建的双光子荧光探针因具有细胞穿透能力强,成像速度快,特异性强,易修饰等特点得到了蓬勃的发展。该文综述了近两年双光子荧光探针成像研究的进展,并对双光子荧光探针的发展前景进行了展望。

Biological imaging technology as the best noninvasive way to study living systems in a natural setting has obtained widespread attention in recent years.Two-photon fluorescence microscope has become the indispensable tool in the field of biological imaging due to the advantages of high signal-to-noise ratio,localized excitation,deeper tissue penetration,less photodamage,prolonged observation time,and better three-dimensional imaging.TP fluorescent probes as the render of fluorescent signals play key roles in two-photon imaging.So far,there are many of the two-photon imaging material have been reported,among which two-photon fluorescent probes based on organic small molecular have been development vigorous owning the good cell permeability,fast imaging,high specificity and easy modification.We summarized the studies of imaging by two-photon fluorescence probe in nearly two years,and the prospect of the development of two-photon fluorescence probes was given.