白藜芦醇对糖尿病性白内障大鼠MDA、SOD和GSH-Px的影响

Effect of resveratrol on MDA,SOD and GSH-Px in diabetic cataract rat

目的探讨白藜芦醇对糖尿病性白内障大鼠的抗氧化作用。方法 Wistar大鼠80只随机分为4组:正常对照组(NC)、糖尿病模型组(DC)、白藜芦醇低剂量组(RL)、白藜芦醇高剂量组(RH)。糖尿病模型组、白藜芦醇低剂量组、高剂量组一次性腹腔注射链脲菌素(STZ 60 mg·kg^(-1))制备糖尿病大鼠模型。成模后,低剂量组每日20 mg·kg^(-1),高剂量组每日100 mg·kg^(-1)白藜芦醇灌胃。裂隙灯显微镜照相机记录晶状体变化。12周实验结束时测各组大鼠晶状体和血清丙二醛(MDA)、超氧化物岐化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)含量。结果糖尿病模型组大鼠晶状体发生了不同程度混浊形成白内障。大剂量白藜芦醇组糖尿病大鼠晶状体混浊程度明显低于白内障糖尿病大鼠。白内障糖尿病大鼠晶状体和血清MDA较正常对照组明显升高(P<0.01),白内障糖尿病大鼠晶状体和血清SOD、GSH-Px较正常对照组晶状体和血清SOD、GSH-Px明显降低(P<0.01)。大剂量白藜芦醇组糖尿病大鼠晶状体和血清MDA含量较白内障糖尿病大鼠明显降低(P<0.01),晶状体和血清SOD、GSH-Px活性较白内障糖尿病大鼠明显升高(P<0.01)。结论白藜芦醇可能通过降低氧化应激损伤延缓糖尿病大鼠白内障的发生发展。

Objective To investigate the antioxidate effect of resveratrol on diabetic cataract rats. Methods Eighty Wistar rats were divided into four groups randomly： control group （NC）, diabetic model group （DC）, low resveratrol dosage of medicine group （RL）, high resveratrol dosage of medicine group （RH）. The model of diabetic rats in diabetic model group, low resveratrol dosage of medicine group and high resveratrol dosage of medicine group were established by using a single intraperitoneal injection with streptozotocin （ STZ 60 mg . kg -1 ）. Resveratrol 20 mg . kg-1 were administered to low resveratrol dosage of medicine group daily, resveratrol 100 mg . kg-1 were administered to high resveratrol dosage of medicine group daily. The changes of lens were recorded by slit lamp microscope camera in four groups. The quantity of malondialdehyde （ MDA）, superoxide dismutase （SOD） and gutathione peroxidase （ GSH- Px） in lens and serum were detected after 12 weeks when rats were killed to end experiment. Results The diabetic cataract rats were replicated successfully. The turbid level of lens in high resveratrol dosage of medicine group was significantly lighter than that in diabetic model group. The expression of MDA in the lens and serum of diabetic cataract rat was increased significantly compared with the control group （P 〈0.01 ）, the expressions of SOD and GSH-Px in the lens and serum of diabetic cataract rat were decreased significantly compared with control group （P 〈 0. 01 ）. The expression of MDA in the lens and serum of high resveratrol dosage of medicine group was decreased significantly compared with that in the lens and serum of diabetic cataract rat （P 〈0. 01 ）, the expression of SOD and GSH-Px in lens and serum of high resveratrol dosage of medicine group was increased significantly compared with that in lens and serum of diabetic cataract rat （ P 〈 0. 01 ）. Conclusion The development of diabetic cataract is prevented by the resveratrol possibly through the amelioration of oxidative stress.