微纳米黄连的制备及其对木材白腐菌的抑菌性

Preparation of micro-nano coptis and its antimicrobial activity on white rot fungi

以中草药黄连为原料,使用物理干法球磨制备微纳米黄连,检测其对木材的防腐效果。采用正交试验方法研究球磨转速、球磨时间、小球数对微纳米黄连制备的影响,并确定最佳制备条件和最小抑菌质量浓度,以激光粒度仪与扫描电子显微镜(SEM)对微纳米黄连的粒度分布与形貌进行检测。结果表明:微纳米黄连粉体较普通黄连粉体有更明显的抑菌效果,经过试验筛选得出制备微纳米黄连粉体的最佳工艺条件为球磨转速280 r/min、球磨时间4 h、小球数60个,此时菌落直径仅为2.91 mm,最小抑菌质量浓度为0.5 mg/m L。SEM与显微粒度检测结果显示,微纳米黄连的主要形貌为块状和不规则的球状,粒度分布均匀且为分散状,粒径宽度和长度大多分布在200~700 nm。

To investigate the antifungal performance of the Chinese herbal medicine on wood, rhizoma coptis was used as a non-toxic and efficient raw material to prepare micro-nano coptis by the method of dry-ball milling. The effects of ball grinding time, ball grinding speed and ball number on the preparation of micro-nano copits were explored through the orthogonal test. The optimal preparation conditions were obtained by the comparison test of antifungal performance. The minimum antifungal mass concentration experiment determined the minimum fungi-static mass concentration of micro-nano coptis. The diameter distribution and morphology of nano-particles was examined by using the laser scattering particle size distribution analyzer and scanning electron microscopy （SEM） respectively. The results showed that the rhizoma coptis particles had more obvious fungistatic effect than the ordinary coptis did. The optimum parameters in the preparation of micro-nano coptis were as follows： the ball mill speed of 280 r/min, ball grinding time of 4 h and ball number of 60. The micro-nano coptis prepared in this condition were distributed with uniform particle size, and the bacteriostatic effect was the best. The minimum diameter of fungal colony was only 2. 91 mm, and the minimum inhibitory mass concentration was 0.5 mg/mL. The results of SEM and microscope analysis showed that the main morphology of micro-nano copits was massive and irregular sphericity, and the particle size dis- tribution was uniform and dispersive. Particle diameter and length were mostly ranged between 200 nm and 700 nm. Had the best bacteriostatic effect, No. 5 powder particle size distributed more uniform than No. 9 powder. The width and height of No. 5 powder ranged between 200 nm and 700 nm, while those of No, 9 powder were between 0. 5 μm and 3.0 μm. Under the same solution concentration, the smaller particle had better bacteriostatic effect.