HPLC-DAD法检测喹乙醇预混剂中违规添加喹烯酮的研究

Determination of Quinocetone into Olaquindox Premix by HPLC-DAD

确证1批次喹乙醇预混剂中的非法添加物。采用HPLC-DAD的谱库匹配,推断出疑似添加物为喹烯酮,从而建立了检测喹乙醇预混剂中违规添加喹烯酮的反相高效液相色谱法(HPLC-DAD)。采用Agilent Eclipse XDB-C18色谱柱(250 mm×4.6 mm,5μm),流动相为甲醇-水(60∶40),流速1.0 m L/min,波长扫描范围为210~400 nm。喹烯酮在1~100μg/m L浓度范围内线性关系良好,r=1.0000;平均回收率为99.4%~99.8%,n=5,RSD在0.8%~1.0%之间。

To identify the unknown adulterated compound in one batch of olaquindox premix in the common course, an unknown compound was observed with characteristic UV spectra. The compound was deduced with the information provided by HPLC - DAD. Results showed that the unknown adulterated compound in this sample was identified as quinocetone. To establish a HPLC - DAD method for the determination of quinocetone in olaquindox premix, the chromatographic column was Agilent Eclipse XDB -C18 （250 mm × 4.6 mm, 5 μm）, the mobile phase consisted of a mixture of a solution of methanol and water （60： 40）. The flow rate was 1.0 mL/min. The scanning wavelength was from 210 to 400 nm. Quinocetone in i - 100 μg/mL concentration range of linear is good （r = 1. 0000）. The average recoveries was 99.4% ~ 99.8% （n = 5） with RSD between 0.8% and 1.0%.