裸鼠人来源性子宫腺肌病造模方法的比较

Comparison of four establishment methods of nude mouse models of human-derived uterine adenomyosis

目的通过不同的造模方法建立裸鼠人来源性子宫腺肌病模型,筛选出理想的造模方法,为研究子宫腺肌病发病机制及相关治疗方案提供理想的动物模型。方法将80只雌性裸鼠随机分为腹腔种植组、皮下种植组、腹腔注射组和皮下注射组,每组20只。各模型组分别采用不同造模方法建立裸鼠人来源性子宫腺肌病模型,术后4周观察移植物的体积及组织形态学。结果腹腔种植组建模成功率95%,皮下种植组建模成功率45%,腹腔注射法及皮下注射法成模率0%。结论腹腔种植法建立子宫腺肌病裸鼠模型成模率高,稳定性良好,是一种理想的人来源性子宫腺肌病动物模型。

Objective The purpose of this study was to compare the adenomyosis models in nude mice generated by four different methods,and to find out an optimal modeling method,and to provide an ideal animal model for exploring pathogenesis and experimental treatment of uterine adenomyosis. Methods 1. 80 female healthy nude mouse were divided randomly into 4 groups： Intraperitoneal implantation group,subcutaneous implantation group,intraperitoneal injection group,and subcutaneous injection group. The transplants were taken for pathological examination at 4 weeks after surgery.Results The success rate of intraperitoneal implantation group was 95%,and that of the subcutaneous implantation group was 45%,while the success rate of intraperitoneal injection group and subcutaneous injection group was 0%. Conclusions Establishment of a nude mouse model of uterine adenomyosis by intraperitoneal implantation method has a high success rate and with good stability,and is an ideal mouse model of human-derived uterine adenomyosis.