辽宁省朝阳市细粒棘球病的基因型分析

Genetic analysis of Echinococcus granulosus strain in Liaoning Province,China

目的分析本院分离到的人源性棘球蚴的基因型,为辽宁省棘球蚴病的分子流行病学提供资料。方法经外科手术方法从患者肝内获取棘球蚴囊,将囊内容物离心沉淀提取DNA,以细粒棘球绦虫线粒体细胞色素氧化酶(cox1)基因为靶基因设计引物进行PCR扩增,对PCR产物进行测序,测序结果应用Blast进行分析,并与GenBank数据库中序列进行比对。结果 cox1扩增产物及测序的结果提示,本例样本与细粒棘球幼的cox1基因型(AF297617)高度相似,差异性为0.1%,基因型属于G1亚型。并与其他国家的25个分离株序列一致。结论线粒体基因组的cox1基因分析是棘球蚴病诊断和分型的有力工具。辽宁地区首次报道了细粒棘球绦虫的基因型为G1亚型,其序列与国内外的多个分离株保持一致,为棘球蚴病的分子流行病学补充了资料。

To analyze the genotype of Echinococcus granulosus found in Liaoning Province, hydatid cyst was taken from the patient＇s liver, DNA of cyst was isolated and mitochondrial coxl gene was sequenced substantially. Sequence results was ana- lyzed by Blast and compared with similar strains in GenBank. Results showed that molecular analysis confirmed the echinococ- cosis by identical sequence of our strain with E. granulosus （AF297617） G1 genotype, with 0. 1% difference. And the se quence of our strain was consistent with 25 other strains in different countries. In conclusion, genotyping of mitochondrial gene coxl is a very useful tool for diagnosis of echinococcosis. We first reported a strain of E. granulosus in Liaoning Province, which provide novel molecular epidemiological data of this zoonosis.