γ-干扰素及外源性吲哚对泌尿生殖道沙眼衣原体生长影响的探讨

Effect of interferon-γ and exogenous indole on the growth of Chlamydia trachomatis

目的探讨外源性吲哚及γ-干扰素对国内沙眼衣原体优势株E-UW-5/Cx型标准株及临床株生长的影响,并与国外优势株D-UW-5/Cx进行比较。方法分别用DMEM-10、DMEM-10加5ng/mL重组人γ-干扰素和DMEM-10加5ng/mL重组人γ-干扰素及50μmol外源性吲哚三种细胞培养液培养沙眼衣原体至48h,甲醇固定后计数包涵体,观察γ-干扰素及外源性吲哚对E型、D型沙眼衣原体标准株及临床株生长的影响。结果 DMEM-10+IFN组衣原体包涵体计数明显低于DMEM-10组和DMEM-10+IFN+IND组,差异有统计学意义,P<0.05;DMEM-10组和DMEM-10+IFN+IND组包涵体计数差异无统计学意义;E型标准株、临床株与D型标准株、临床株之间差异无统计学意义,P>0.05。结论在IFN-γ作用下,沙眼衣原体的生长繁殖受到明显抑制,加入外源性吲哚后,泌尿生殖道沙眼衣原体可以逃逸IFN-γ介导的清除作用,恢复其感染活力。

We investigated the effects of 7-interferon and exogenous indole on the growth of domestic dominant standard strains and clinical strains of Chlamydia trachomatis E-UW-5/Cx, and compared with the dominant strains of D-UW-5/Cx a- broad. We used DMEM-10, DMEM-10 containing 5 ng/mL recombinant human interferon gamma （referred to as DMEM-10q- IFN） and DMEM-10 containing 5 ng/mL recombinant human interferon gamma and 50 μM exogenous indole （referred to as DMEM-10＋IFN＋IND） to culture C. trachornatis, and then we fixed it with methanol to count inclusions after 48 hours, ohserving the influence of r-interferon and exogenous indole on the growth of C. trachomatis standard strains（E, D） and clinical strains. Results showed that the count of Chlamydia inclusion bodies in DMEM 10＋IFN group was significantly lower than others （P〈0.05）; no significant difference was found （P〉0.05） between the count of DMEM-10 group between DMEM-10＋ IFN＋IND group. There were no significant difference between the E and D standard or clinical strains （P〉0.05）. Under the effect of IFN 7, the growth of domestic dominant strain E-UW-5/Cx C. trachomatis was significantly inhibited. After adding exogenous indole, C. trachomatis can escape the scavenging activity of IFN-γ to restore the infection vitality.