43株甘肃临床分离非结核分枝杆菌分类鉴定

Species identification on 43 clinical non-tuberculosis Mycobacteria isolates from Gansu Province,China

目的了解甘肃当前临床流行的非结核分枝杆菌(NTM)的病原谱特点及其主要NTM种类,为指导临床诊疗、有效防治NTM肺病提供参考依据。方法收集2012年~2014年来源于甘肃省不同地区的875株临床分离分枝杆菌菌株,经PNB/TCH方法初步鉴定为疑似NTM菌株,采用16SrRNA基因测序技术进行菌种鉴定。结果 875株分枝杆菌临床分离菌株中分离出疑似NTM菌株46株。经16SrRNA基因序列分析鉴定,43株为NTM,3株为诺卡氏菌。43株NTM菌株分别为胞内分枝杆菌、堪萨斯分枝杆菌、塞内加尔分枝杆菌、鸟分枝杆菌、戈登氏分枝杆菌、楚尔盖分枝杆菌、罕见分枝杆菌、偶然分枝杆菌和脓肿分枝杆菌。其中,胞内分枝杆菌有31株,占总NTM菌株数的72.09%。结论甘肃省非结核分枝杆菌群以胞内分枝杆菌为主,应用16SrRNA基因序列分析鉴定方法能准确鉴定出NTM菌种,为临床诊治提供依据。

To understand pathogen spectrum of nontuberculosis Mycobacteria （NTM） and the dominant NTM in Gansu Province and provide the scientific basis for the effective prevention and treatment of NTM diseases, 875 Mycobacteria isolates were collected from 2012 to 2014 in Lanzhou Pulmonary Hospital, NTM species were identified by means of PNB/TCH differ- entiate medium and 16S rRNA gene sequence analysis respectively. Forty-six isolats of NTM were identied from 8？5 PNB/ TCH. Then with 16S rRNA gene sequence analysis,the NTM strains were identified to 3 strains of Nocadia and 43 strains of NTM, including M. intracellulare, M. kansasii, M. avium, M. senegalense, M. gordonae, M. szulgai, M. peregrinu- mand M. fortuitum. Among them, there were 31 strains of M. intracellulare, which accounted for 72.09% of the total num- ber of NTM strains. The dominant nontuberculosis Mycobacteria in Gansu Province were mainly M. intracellulare. The appli- cation of molecular biology can rapidly and accurately identify the species of nontuberculosis Mycobacteria, and can provide rel- evant evidence for clinical diagnosis and therapy.