胶体钯-石墨炉原子吸收直接测定枸杞酒中硒含量的方法研究

Direct Determination of Selenium Content in Wolfberry Wine by Graphite Furnace Atomic Absorption Spectrometry with Colloid Palladium

硒是枸杞酒的重要功效成分之一,需要对枸杞酒中的硒含量进行测定。本文采用胶体钯-石墨炉原子吸收直接测定枸杞酒中的硒含量。不经过繁琐的消解过程,将酒样酸化和稀释后直接上机测定,在石墨炉升温过程中去除酒样中的有机杂质,这大大简化了测定步骤。对此法的升温程序进行优化,然后在优化条件下测定硒含量。结果表明,添加胶体钯作为基体改进剂的优化升温程序为:干燥温度110℃、时间40 s,灰化温度1100℃、时间20 s、升温速率10℃/s,原子化温度2200℃;此法测定硒标准曲线方程为:Y=0.000024x^2+0.0041x+0.017,相关系数R2=0.9988;检出限为26.74 pg;测定枸杞酒中硒含量为14.52μg/L,RSD值<10%,加标回收率为102.60%。因此,胶体钯-石墨炉原子吸收测定枸杞酒中硒含量的方法准确可靠,简单可行。

Selenium（Se） is one of the important functional components in wolfberry wine;hence,it is necessary to determine the Se content.In this study,the Se content in wolfberry wine was directly determined by graphite furnace atomic absorption spectrometry（GFAAS） with colloid palladium.The tedious digestion process was avoided,the wine sample was directly determined after acidification and dilution,and organic impurities in the wine sample were removed during the heating process of the graphite furnace,which greatly simplified the measurement process.The heating program was optimized,and the Se content was determined under the optimized conditions.The results showed that with colloid palladium as a matrix modifier,the optimal heating program was as follows：drying at 110 ℃ for 40 s,ashing at 1100 ℃ for 20 s with a heating rate of 10 ℃/s,and atomization at 2200 ℃.The standard curve for the determination of Se content by this method was obtained：y= 0.000024x-2＋0.0041x＋0.017,R-2=0.9988.The detection limit was 26.74 pg,the Se content in wolfberry wine was determined as 14.52 μg/L（RSD10%）,and the recovery was 102.60%.Therefore,with colloid palladium as the matrix modifier,the GFAAS method is accurate,reliable,simple,and feasible for the direct determination of Se content in wolfberry wine.