超声引导经食道起搏电极定位建立大鼠快速心房起搏房颤模型的实验研究

Establishment of atrial fibrillation model in rats induced by rapid atrial pacing via ultrasound-guided esophageal pacing catheter placement

目的探讨应用超声引导经食道起搏电极定位建立大鼠快速心房起搏房颤模型的可行性。方法分别应用超声和食道心电图(心房A波振幅大小和形态)引导经食道起搏电极定位建立大鼠快速心房起搏诱导房颤模型,即为超声引导定位组(US组)和食道心电图引导定位组(ECG组),比较两组的房颤诱导成功率、平均房颤持续时间、平均定位耗时、平均起搏阈值及无关肌肉刺激评分。结果与ECG组比较,US组房颤诱导成功率高(80%vs.60%),平均定位耗时短[(2.3±0.5)min vs.(5.3±0.9)min],平均起搏阈值低[(0.350±0.048)mV vs.(0.570±0.057)mV],无关肌肉刺激评分小[(0.79±0.58)vs.(1.32±0.46)分],差异均有统计学意义(均P<0.05);两组平均房颤持续时间比较差异无统计学意义。结论超声引导经食道起搏电极定位建立大鼠快速心房起搏房颤模型有效、安全。

Objective To investigate the feasibility of ultrasound-guided esophageal pacing catheter placement for establishing atrial fibrillation model in rats.Methods Establish atrial fibrillation model in rats via rapid atrial pacing with the guidance of ultrasound and esophageal electrocardiogram（atrial A-wave amplitude and form）,and the rats were divided into ultrasound-guided（US）group and esophageal electrocardiogram-guided（ECG）group,respectively.Then a comparison was made between US and ECG group in induced efficacy of atrial fibrillation,mean duration of atrial fibrillation,mean catheter placement time,mean pacing threshold and less extra muscular stimulation.Results Compared with ECG group,ultrasound-guided esophageal pacing catheter placement resulted in higher atrial fibrillation rate（80% vs.60%）,shorter mean catheter placement time[（2.3±0.5）min vs.（5.3±0.9）min],lower mean pacing threshold[（0.350±0.048）mV vs.（0.570±0.057）mV]and less extra muscular stimulation[（0.79±0.58）min vs.（1.32±0.46）min],there were significant differences among these indexes（all P〈0.05）.While there was no significant difference of mean duration of atrial fibrillation between two groups.Conclusion Ultrasound-guided esophageal pacing catheter placement is an effective and safe method for the establishment of atrial fibrillation model in rats.