儋州鸡不同产蛋时期生殖激素分泌规律的研究

Study on the Secretion of Reproductive Hormone in Danzhou Chicken at Different Laying Period

为研究儋州鸡不同产蛋时期生殖激素的分泌规律,采用ELISA方法对儋州鸡母鸡开产前期、开产期、产蛋高峰期及产蛋后期4个时期血液中促卵泡素(FSH)、促黄体生成素(LH)、催乳素(PRL)、孕酮(P)、雌二醇(E_2)的浓度进行测定。结果显示,开产期与产蛋高峰期FSH与LH浓度极显著高于开产前期和产蛋后期(P<0.01);不同产蛋时期血液中PRL浓度呈上升趋势,且开产前期和开产期PRL浓度显著低于产蛋后期(P<0.05);不同产蛋时期血液中E_2浓度呈下降趋势,且产蛋后期E_2浓度显著高于开产前期和开产期(P<0.05),极显著高于产蛋高峰期(P<0.01);开产期与产蛋后期P的浓度较高,均显著高于开产前期(P<0.05),且极显著高于产蛋高峰期(P<0.01)。研究表明,儋州鸡在不同产蛋时期血液中生殖激素含量水平有不同的变化,开产期血液中FSH、LH及P浓度较开产前期明显升高;产蛋高峰期血液中FSH与LH的浓度高于其他时期;产蛋后期血液中PRL、E_2、P的浓度较产蛋高峰期明显上升,且负反馈调节FSH与LH的分泌量。

To investigate the secretion of reproductive hormone of Danzhou chicken at different laying period, enzyme-linked immunosorbent assay （EL1SA）was applied for the determination of follicle-stimulating hormone （FSH）, luteotropic hormone （LH）,prolactin （PRL）,progesterone （P）, and estradiol （E2）in blood at open prenatal period,open perinatal period, peak period and late laying period. The results showed that FSH and LH at open perinatal and peak period were significantly higher than that at open prenatal period and late laying period （P〈0.01）;the level of PRL was escalated from open prenatal period to late laying period （P〈0.05）;the level of E2 was gradually reduced from open prenatal period to peak period,and then significantly increased at late laying period （P〈0.01）. P had a high concentration at open perinatal period and late laying period,and significantly higher than that at open prenatal period （P〈0.01）. It was concluded that there were different changes in the levels of reproductive hormones in the blood of Danzhou chicken. The FSH,LH and P concentrations at open perinatal period were significantly higher than those at open prenatal period. The FSH and LH concentrations in the blood at the peak of laying period were higher than those at other periods.The concentrations of PRL,E2 and P in the blood at the later of laying period was significantly higher than those at the peak period,and there was a negative feedback regulation of FSH and LH secretion.