参芪养心汤对心力衰竭大鼠心功能的保护作用及相关机制研究

Protective Effect and Mechanism of Shenqi Yangxin Tang for Heart Failure Rats

目的:研究参芪养心汤对心力衰竭大鼠心功能的保护作用及相关机制。方法:将40只雄性SD大鼠随机分为四组:假手术组,心衰模型组,参芪养心汤低剂量组,参芪养心汤高剂量组。通过腹主动脉缩窄法制作慢性心力衰竭模型,给予不同剂量的参芪养心汤干预,通过检测血清脑钠肽(BNP)及血流动力学变化评估心功能,免疫组化法观察心肌组织病理学改变,TUNEL法检测心肌细胞凋亡,Western blotting检测凋亡相关蛋白Bcl-2及Bax的表达。结果:与假手术组比较,心衰模型组大鼠BNP水平升高,大鼠左室收缩压(LVSP)、左心室内压最大上升速率(+d P/dt_(max))和左心室内压最大下降速率(-d P/dt_(max))均显著降低,左心室舒张末压(LVEDP)升高,心肌细胞凋亡量数量显著增加,促凋亡蛋白Bax表达量增加,抗凋亡蛋白Bcl-2表达降低,差异均有统计学意义(P<0.05),说明心衰模型造模成功。与心衰模型组比较,参芪养心汤低剂量组和高剂量组大鼠BNP水平均显著降低,LVSP、+d P/dt_(max)和-d P/dt_(max)均显著升高,LVEDP均降低,大鼠心肌细胞凋亡数量显著减少,Bax表达下降,Bcl-2表达回升,差异均有统计学意义(P<0.05)。与假手术组比较,参芪养心汤低剂量组和高剂量组大鼠BNP水平均仍较高,差异均有统计学意义(P<0.05)。结论:参芪养心汤能改善心力衰竭大鼠心功能,抑制心肌细胞凋亡。

Objective：To study protective effect and related mechanism of Shenqi Yangxin tang for heart failure rats.Methods：Selected 40 male SD rats and divided them into four groups：sham operation group,heart failure model group,low dose Shenqi Yangxin tang group and high dose Shenqi Yangxin tang group.Establishing chronic heart failure model with abdominal aortic banding method,and giving Shenqi Yangxin tang intervention of different doses.Evaluated cardiac function by detecting change of serum brain natriuretic peptide（BNP） and hemodynamics,observed change of histopathology of myocardium with immunohistochemistry,detected cardiomyocyte apoptosis with terminal deoxynucleotidyl transferasemediated dUTP- biotin nick end labeling assay（TUNEL）,and apoptosis- related proteins Bcl- 2 and Bax with Western blotting.Results：Comparing with sham operation group,BNP levels of heart failure model group were increased,left ventricular systolic pressure（LVSP）,maximal ascending rate of internal pressure of left ventricle（＋dP/dtmax）,and maximum descending rate of interal pressure of left ventricular（-dP/dtmax） of rats were all decreased significantly,left ventricular end diastolic pressure（LVEDP） was increased,the number of myocardial apoptosis was increased significantly,expression of proapoptotic protein Bax was increased,and the expression of anti- apoptotic protein Bcl- 2 was decreased,the difference being significant（P〈0.05）,which suggested the successful modeling of heart failure model.Compared with heart failure model group,BNP levels of the low dose and high dose Shenqi Yangxin tang group were decreased significantly,LVSP,＋dP/dtmaxand- dP/dtmax were all increased,LVEDP levels were both decreased,the number of myocardial apoptosis of rats was decreased significantly,expression of Bax was decreased,expression of Bcl- 2 was returned,differences being significant（P〈0.05）.Comparing with sham operation group,BNP levels of the low dose and high dose Shenqi Yangxin tang group were still high,differences being significant（P〈0.05）.Conclusion：Shenqi Yangxin tang can improve cardiac function and inhibit cardiomyocyte apoptosis of rats with heart failure.