福建省羊支原体性肺炎分子流行病学研究

A Molecular Epidemiological Study on Mycoplasma Pneumonia of Sheep and Goats in Fujian

为了解福建羊支原体性肺炎和山羊传染性胸膜肺炎的分子流行情况,于2014-2016年从福建省6个市采集到疑似羊支原体性肺炎或山羊传染性胸膜肺炎病料61份,用支原体目引物、绵羊肺炎支原体、丝状支原体山羊亚种、山羊支原体山羊肺炎亚种、山羊支原体山羊亚种、精氨酸支原体、莱氏无胆甾原体和无乳支原体的特异引物,通过PCR方法进行检测。挑取部分PCR阳性病料进行支原体分离和纯化,用16SrRNA基因通用引物进行克隆测序。结果显示61份样品中检出绵羊肺炎支原体45份(73.77%),检出丝状支原体山羊亚种4份(6.56%),检出莱氏无胆甾原体4份(6.56%),检出精氨酸支原体2份(3.28%)。该研究结果表明福建省发生的类传染性胸膜肺炎是以绵羊肺炎支原体为主的羊支原体性肺炎,为福建省羊支原体性肺炎的有效防治提供了科学依据。

The molecular epidemiology on the mycoplasma pneumonia of sheep and the contagious caprine pleuropneumonia of goats in Fujian were investigated. A total of 61 samples from the goats with suspected infection of the diseases were collected from 6 regions in the province during 2014- 2016. PCR with the mycoplasmatales primer as well as the specific primers for Mycoplasma ovipneumoniae, Mycoplasma mycoides subsp, capri, Mycoplasma capricolum Subsp. Capripneumonia, Mycoplasrna capricolum suhsp, capri, Mycoplasma arginin, Acholeplasma laidlawii and Mycoplasma agalactiae were applied for the detection. Part of the PCR positive clinical samples were selected to isolate Mycoplasma for subsequent cloning and sequencing based on their 16S rRNA. The detection rates on M. ovipneumoniae, M. mycoides subsp, capri, A. laidlawii and M. arginine were 73. 77% （45/61）, 6.56% （4/61）, 6.56% （4/61） and 3.28% （2/61）, respectively. The results suggested that the suspected contagious caprine pleuropneumonia in the province were mycoplasma pneumonia of sheep and goats caused mainly by M. ovipneumoniae. The finding would lead to effective prevention and treatment measures for the diseases.