强筋壮骨祛风合剂对腰椎间盘退变AQP1和AQP5表达的干预作用

Effect of Qiangjinzhuangguqufeng Mixture on the Expression of AQP1 and AQP5 in Lumbar Intervertebral Disc Degeneration

目的:观察强筋壮骨祛风合剂对腰椎间盘退变水通道蛋白1(AQP1)和水通道蛋白5(AQP5)表达的干预作用。方法:将新西兰大白兔腰椎穿刺建立腰椎间盘退变模型,用RT-PCR及免疫组化检测AQP1和AQP5在椎间盘组织中的表达,观察模型大白兔给药6周后的变化。结果:1)RT-PCR检测:(1)各组AQP1表达比较,假手术组与空白对照组差异无统计学意义(P>0.05),模型空白组、腰痛宁组、强筋壮骨祛风合剂组与空白对照组差异有统计学意义(P<0.05),空白对照组、假手术组、腰痛宁组、强筋壮骨祛风合剂组与模型空白组差异有统计学意义(P<0.05)。(2)各组AQP5表达比较,假手术组与空白对照组差异无统计学意义(P>0.05),模型空白组、腰痛宁组、强筋壮骨祛风合剂组与空白对照组差异有统计学意义(P<0.05),空白对照组、假手术组、腰痛宁组、强筋壮骨祛风合剂组与模型空白组差异有统计学意义(P<0.05)。2)免疫组化试剂检测:(1)各组AQP1表达比较,造模成功后,模型空白组椎间盘上AQP1阳性面积与空白对照组、假手术组差异有统计学意义(P<0.05),空白对照组和假手术组差异无统计学意义(P>0.05),其余组之间差异均有统计学意义(P<0.05)。(2)各组AQP5表达比较,造模成功后,模型空白组椎间盘上AQP5阳性面积与空白对照组、假手术组差异有统计学意义(P<0.05),空白对照组和假手术组差异无统计学意义(P>0.05),其余组之间差异均有统计学意义(P<0.05)。结论:强筋壮骨祛风合剂对AQP1及AQP5的调节作用是其疗效机制之一,具有改善腰椎间盘退变的作用。

Objective： To observe the intervention mechanism of Qiangjinzhuangguqufeng mixture on the expression of aquaporinl （AQP1）and aquaporin5（AQP5）in lumbar intervertebral disc degeneration. Methods：The lumbar intervertebral disc degeneration model was established by lumbar puncture in New Zealand white rabbits. The expression of AQP1 and AQP5 in intervertebral disc was detected by RT-PCR and immunohistochemistry. The changes of the model rabbits were observed 6 weeks after the adminis- tration. Results： 1）RT-PCR detection： （1）The expression of AQP1 in each group was compared. There was no difference in the ex- pression of AQP1 between the sham operation group and the blank control group（P^0.05）. There were difference in the expres- sion of AQP1 among the model blank group, the Yaotongning group, the Qiangjinzhuangguqufeng mixture group and the blank control group（P（0.05）. （2）And there were difference among the blank control group, the sham operation group, the Yaotongn- ing group,the Qiangjinzhuangguqufeng mixture group and the model blank group（P〈0.05）. The expression of AQP5 in each group was compared. There was no difference in the expression of AQP5 between the sham operation group and the blank control nohistochemically detection：（1）The expression of AQP1 in each group was compared. After successful modeling, the positive area of AQP1 in intervertebral discs in the model blank group was different from that in the blank control group and the sham opera- tion group（P〈0.05） ,but there was no difference between the blank control group and the sham operation group（P〉0. 05） ,the rest had statistically significant difference（P〈0.05）. The expression of AQP5 in each group was compared. Compared with blank control group and the sham operation group, the positive area of AQP5 in intervertebral discs in model control group was different （P〉0. 05 ）, but there was no significant difference between the blank control group and the sham operation group（P〉0.05）, the rest had statistical significance（P〈0.05）. Conclusion： Qiangjinzhuangguqufeng mixture on the regulation of AQP1 and AQP5 is one of the therapeutic mechanisms, which can improve the degeneration of lumbar intervertebral disc.