药品微生物实验室高压蒸汽灭菌锅的规范化使用

Standardizing Use of High pressure steam autoclaves in Microbiology Laboratory

高压蒸汽灭菌锅是药品微生物实验室培养基、实验器具、阳性废弃物最常用的、有效的灭菌设备。高压蒸汽灭菌锅从验证到灭菌状态的维护应严格遵守操作规范。为了确保高压蒸汽灭菌锅的有效使用,必须从安装确认、操作确认、工艺确认等方面进行好验证工作,在使用过程中要用参数监测、化学监测、生物监测等手段对灭菌质量有效评价。

Autoclave is most effectively sterilizing equipment commonly used in placing culture medium and positive contaminants. But it is necessary to strictly conform to the standards in validation of autoclave and the maintenance of its sterilizing function. In order to ensuring the effective use of autoclave, the validations in installation, operation and process are very important. Thus the methods of parameter monitor, chemical monitor and biological monitor should be applied in practice to make effective evaluation.