抑制IGFBP2、IGFBP3在卵巢肿瘤中的表达对卵巢癌的迁移、侵袭的影响

Effects of IGFBP2 and IGFBP3 expression in ovarian tumors on migration,invasion of ovarian cancer

目的研究抑制IGFBP2、IGFBP3在卵巢肿瘤中的表达对卵巢癌的迁移侵袭的影响。方法采用siRNA干扰IGFBP2、IGFBP3在卵巢癌细胞株SKOV3中的表达,CCK-8试剂盒检测SKOV3的增殖情况,流式细胞术检测SKOV3的凋亡情况,transwell实验、细胞划痕愈合实验检测SKOV3的迁移与侵袭;CCK-8法检测不同浓度顺铂(5、10、20μg/m L)对SKOV3细胞生长的影响。结果采用siRAN干扰IGFBP2、IGFBP3在卵巢癌SKOV3中表达后,与对照组相比SKOV3细胞增殖能力下降,凋亡增加,迁移与侵袭功能下降。结论抑制IGFBP2、IGFBP3在卵巢肿瘤中的表达对卵巢癌的迁移与侵袭起一定的抑制作用。

Objective To study the effect on ovarian tumor migration invasion after inhibition the expression of IGFBP2, IGFBP3. Methods siRAN interference IGFBP2, IGFBP3 expression in ovarian cancer cell lines SKOV3, SKOV3 proliferation detected by CCK-8 kits,SKOV3 apoptosis detected by flow cytometry, SKOV3 migration and invasion detected by transwell experiment and scratched cell healing detection; CCK-8 method detected survival after treated different concentrations of cisplatin （5, 10, 15, 20 ug/mL）. Results The proliferation ability of SKOV3 dropped and apoptosis increased after treated siRAN IGFBP2, IGFBP3 compared with the control group , migration and invasion function decline, resistance level to improve greatly. Conclusions The expression of IGFBP2, IGFBP3 in ovarian cancer affected migration and invasion.