摘要
Background： Lycopene（LYC） is a natural carotenoid with powerful reactive oxygen species（ROS） scavenging activities. The aim of this study was to investigate if lycopene has the ability to reverse ROS-mediated alterations to the motility, viability and intracellular antioxidant profile of bovine spermatozoa subjected to ferrous ascorbate（Fe AA）. Spermatozoa were washed out of fresh bovine semen, suspended in 2.9 % sodium citrate and subjected to LYC treatment（0.25, 0.5, 1 or 2 mmol/L） in the presence or absence of Fe AA（150 μmol/L Fe SO4 and 750 μmol/L ascorbic acid） during a 6 h in vitro culture. Spermatozoa motion characteristics were assessed using the Sperm Vision？computer-aided sperm analysis（CASA） system. Cell viability was examined with the metabolic activity（MTT） assay,ROS generation was quantified via luminometry and the nitroblue-tetrazolium（NBT） test was applied to quantify the intracellular superoxide formation. Cell lysates were prepared at the end of the in vitro culture to investigate the intracellular activity of superoxide dismutase（SOD）, catalase（CAT）, glutathione peroxidase（GPx） as well as the concentrations of glutathione（GSH） and malondialdehyde（MDA）.Results： FeA A treatment led to a reduced spermatozoa motility（P 〈 0.001）, viability（P 〈 0.001） and a decline of the antioxidant capacity of spermatozoa（P 〈 0.001） but increased the ROS generation（P 〈 0.001）, superoxide production（P 〈 0.001） and lipid peroxidation（P 〈 0.001）. LYC administration resulted in a preservation of the spermatozoa motion parameters（P 〈 0.001）, mitochondrial activity（P 〈 0.001） and antioxidant characteristics（P 〈 0.001 with respect to SOD;P 〈 0.01 in relation to CAT; P 〈 0.05 as for GPx and GSH） with a concentration range of 1 and 2 mmol/L LYC revealed to be the most effective.Conclusions： Our results suggest that LYC exhibits significant ROS-scavenging and antioxidant properties which may prevent spermatozoa alterations caused by oxidative stress, and preserve the functionality of male reproductive cells.
Background： Lycopene（LYC） is a natural carotenoid with powerful reactive oxygen species（ROS） scavenging activities. The aim of this study was to investigate if lycopene has the ability to reverse ROS-mediated alterations to the motility, viability and intracellular antioxidant profile of bovine spermatozoa subjected to ferrous ascorbate（Fe AA）. Spermatozoa were washed out of fresh bovine semen, suspended in 2.9 % sodium citrate and subjected to LYC treatment（0.25, 0.5, 1 or 2 mmol/L） in the presence or absence of Fe AA（150 μmol/L Fe SO4 and 750 μmol/L ascorbic acid） during a 6 h in vitro culture. Spermatozoa motion characteristics were assessed using the Sperm Vision？computer-aided sperm analysis（CASA） system. Cell viability was examined with the metabolic activity（MTT） assay,ROS generation was quantified via luminometry and the nitroblue-tetrazolium（NBT） test was applied to quantify the intracellular superoxide formation. Cell lysates were prepared at the end of the in vitro culture to investigate the intracellular activity of superoxide dismutase（SOD）, catalase（CAT）, glutathione peroxidase（GPx） as well as the concentrations of glutathione（GSH） and malondialdehyde（MDA）.Results： FeA A treatment led to a reduced spermatozoa motility（P 〈 0.001）, viability（P 〈 0.001） and a decline of the antioxidant capacity of spermatozoa（P 〈 0.001） but increased the ROS generation（P 〈 0.001）, superoxide production（P 〈 0.001） and lipid peroxidation（P 〈 0.001）. LYC administration resulted in a preservation of the spermatozoa motion parameters（P 〈 0.001）, mitochondrial activity（P 〈 0.001） and antioxidant characteristics（P 〈 0.001 with respect to SOD;P 〈 0.01 in relation to CAT; P 〈 0.05 as for GPx and GSH） with a concentration range of 1 and 2 mmol/L LYC revealed to be the most effective.Conclusions： Our results suggest that LYC exhibits significant ROS-scavenging and antioxidant properties which may prevent spermatozoa alterations caused by oxidative stress, and preserve the functionality of male reproductive cells.
基金
supported by the Research Center Agro Bio Tech built in accordance with the project Building Research Centre “Agro Bio Tech” ITMS 26220220180
by the VEGA Project of the Scientific Grant Agency of the Ministry of Education of the Slovak Republic and of the Slovak Academy of Sciences no. 1/0857/14
by the Slovak Research and Development Agency Grant no. APVV-0304-12
