大豆抗原蛋白β-conglycinin线性表位及其不同种属动物过敏血清结合能力比较

Comparison of binding capacity of soybean β-conglycinin linear epitopes and allergic serum of different species animals

试验通过生物信息学方法预测β-conglycinin线性表位,综合已发表文献筛选出四段具有代表性氨基酸序列合成短肽,采用ELISA和点杂交法检测表位肽段与仔猪、犊牛、兔三种不同种属动物过敏血清结合程度差异。结果表明:通过生物信息学方法预测合成的肽段均为β-conglycinin表位肽;三种动物过敏血清与四段表位肽结合能力存在显著差异,其中四段表位肽与仔猪、犊牛过敏血清结合能力显著高于兔过敏血清(P<0.05);同一动物与四段不同肽段结合敏感程度差异显著(P<0.05),兔过敏血清与肽段β1结合能力最强,仔猪过敏血清与α1肽段结合能力最强,犊牛过敏血清与肽段β2肽段结合能力最强。结果为寻找β-conglycinin最佳优势表位提供理论支持,同时为揭示大豆抗原蛋白种属间致敏差异机理奠定基础。

The binding capacity of β-conglycinin linear epitopes and three different species（rabbit,piglets, calf） allergic serum was compared in this study. The linear epitopes were firstly predicted by bioinformatics method, then the four peptides with linear epitopes were selected and synthesized in terms of the predicted results and relative reports. The four peptides were identified with different allergic serum by ELISA and Dot blot. Result showed that the peptides synthesized by bioinformatics method were all β-conglycinin peptides;Another finding was that the binding ability of the different species animals and the same peptides have the significant difference（P〈0.05）, four peptides and piglets, calf allergies serum bining ability significantly stronger than rabbit serum; Four different peptides bining with the allergic serum of the same animal showed significant difference（P〈0.05）, the allergic rabbit serum presented the strongest combined ability with the peptides β1; the allergic piglets serum presented the strongest combined ability with the peptides α1; the allergic calf serum presented the strongest combined ability with peptides β2. The result provided theoretical support for select β-conglycinin best advantage peptide, and revealed the mechanism of soybean protein antigen sensitization differences between species.