海南汉族人群MICB等位基因多态性与类风湿性关节炎相关性研究

Study on the association between polymorphism of MICB gene and rheumatoid arthritis in Hainan Han Chinese population

目的研究海南汉族人群MHCI类链相关基因B（MHCclassIchain-relatedgeneB，M1CB）等位基因多态性与类风湿性关节炎（rheumatoidarthritis，RA）的相关性。方法用世界卫生组织推荐的标准盐析法提取外周静脉血DNA，采用PCR-序列特异性引物（PCRsequencespecificprimer，PCR-SSP）和PCR产物直接测序分型（PCR-sequencebasedgenotyping，PCR-SBT）方法对样本MICB基因的多态性进行检测分析。结果RA患者及对照组中共有10种MICB等位基因被检测出，两组中MICB。005：02等位基因频率分布最高，其频率为52．6％比42．0％，但MICB＊002等位基因频率在RA组明显低于对照组（13．1％比25．5％），差异具有统计学意义（P〈0．05），MICB＊014等位基因频率在RA组亦明显低于对照组（8．1％比15．3％），差异具有统计学意义（P〈0．05）。结论MICB＊002和MICB＊014等位基因与RA的易感性之间存在负相关，可能是RA的保护性基因。

Objective To study on the polymorphism of MICB and its correlation with rheumatoid ar- thritis（RA） in Hainan Han Chinese population. Methods The DNA was extracted from peripheral blood u- sing the standard salt precipitation method recommended by world health organization. PCR-SSP and PCR-SBT were used to analyze the gene polymorphism of M1CB. Results 10 MICB alleles was found in RA patients and control group. Among the tested individuals ,MICB ＊ 005：02 showed relatively high allele frequency. Its frequen- cy for the patient group and control group was 52.6% vs 42.0%. The allele frequency of MICB ＊ 002 in RA group （ 13.1% ） was much lower than that of control group （25.5 % ）, which was statistically significant （Pc 〈 0. 05）. The allele frequency of MICB ＊ 014 in RA group（8.1% ） was significantly lower than that of control group（ 15.3% ） ,which was statistically significant（ Pc 〈 0.05 ）. Conclusions MICB ＊ 002 and MICB ＊ 014 al- leles were negatively correlated with the susceptibility of RA and their might be serve as protective factors.