紫砂芸豆花色苷的超声/微波协同提取研究及其组成分析

Research on Ultrasonic/Microwave Synergistic Extraction of Anthocyanins from Purple Kidney Bean and Structure Identification

以东北紫砂芸豆为原料,采用单因素试验和响应面优化试验研究超声/微波协同提取紫砂芸豆中花色苷的工艺参数,并采用液质连用色谱法对提取物进行不同浓度乙醇洗脱,进行组分分析。结果表明,超声/微波协同提取紫砂芸豆花色苷的有效工艺参数为:微波功率251 W,液料比13.7 mL/g,提取时间24.9min。不同浓度乙醇洗脱液的色谱分析发现,提取物中主要有5种花色苷物质,其中天竺葵素-3-葡萄糖苷、芍药素-3-葡萄糖苷含量较高,且主要集中在40%乙醇洗脱液中。本研究为大孔树脂纯化紫砂芸豆花色苷提供了参考,为紫砂芸豆花色苷的提取加工及工业应用提供依据。

In this paper, purple kidney bean varieties from northeast of China was taken as the raw material, the ultrasonic/microwave synergistic extraction process of anthocyanins from purple bean were studied, and the struc- ture was identified. As indicated in the results, the optimum ultrasonic/microwave synergistic extraction process parameters of anthocyanins from purple kidney bean were shown as following： microwave power 251 W, liquid to solid ratio 13.7 mL/g, extraction time 24.9 min. Chromatographic analysis of ethanol eluent with different concentration showed that there were mainly five kinds of anthocyanins substances, in which pelargonidin 3 - glucoside, peony - 3 - glucoside were higher by of different concentration ethanol elution liquid quality analysis, pelargonidin3 - glucoside, peony prime3 - glucoside mainly concentrated in 40% ethanol eluent. The research may provide reference for anthocyanin purification with macroporous resin, and as theory base for anthocyanin process and its industrial application.