野生大豆与栽培大豆抗旱性对接种丛枝菌根真菌的响应

Responses of Drought Resistance from Wild and Cultivated Soybeans to Arbuscular Mycorrhizal Fungi Inoculation

采用盆栽试验,分别对栽培大豆和野生大豆接种AM真菌进行研究,同时设不接种对照。接种和不接种处理均下设2种水分处理:植物生长期间全程正常供水和前期正常供水后期干旱处理,研究了水分胁迫下接种丛枝菌根(AM)真菌对栽培大豆和野生大豆生理生化特性的影响及菌根水孔蛋白基因GintAQPF1和GintAQPF2表达量的变化。结果表明:干旱胁迫下接种AM真菌能刺激野生大豆和栽培大豆根系伸长生长,对茎秆的促进作用并不明显;干旱胁迫下脯氨酸含量与过氧化氢酶(CAT)活性显著高于未接种对照组,其中接种AM真菌的野生大豆脯氨酸含量、CAT活性增幅最大;未接种AM真菌的栽培大豆与野生大豆丙二醛(MDA)含量与相应接种AM真菌组比显著增加;接种AM真菌的野生大豆与栽培大豆中GintAQPF1和GintAQPF2表达量均显著增加,其中野生大豆GintAQPF2表达量显著高于栽培大豆。说明接种AM真菌能够促进脯氨酸积累,增加CAT活性,提高GintAQPF1和GintAQPF2基因表达,刺激根系的伸长生长。

A greenhouse pot - experiment was conducted in this study to investigate the effects of inoculation with arbuscular mycorrhi- zal （AM） fungi on physiological and biochemical characteristics and relative expression of AM fungi aquaporin genes （GintAQPF1 and GintAQPF2） of wild and cultivated soybean under water stress. Wild and cultivated soybeans were inoculated with AM fungi respectively, and uninoculated treatment as the control. Two water treatments （drought stress and normal irrigation condition） were applied to both the AM and non - AM plants. The results showed that ： AM fungi inoculation stimulated the root growth of wild and cultivated soybean under drought stress, while the stimulation to stems was not significant. The content of free proline and the activity of Catalase （CAT） in AM soybeans were significantly higher than that in non -AM soybean under drought stress condition, and the highest in- crease was the AM wild soybean; however, the content of Malondialdehyde （MDA） showed the opposite trend. The expression levels of GintAQPF1 and GintAQPF2 in AM soybeans were increased significantly, and the expression level of GintAQPF2 in wild soybean was significantly higher than that in cultivated soybean. These suggest that AM symbiosis could increase the accumulation of proline and the activity of CAT, improve the expression level of GintAQPF1 and GintAQPF2, and thus stimulate the growth of soybean roots.