摘要
目的探讨miR-31表达与Dock180表达的相关性,以及miR-31通过结合Dock180对乳腺癌细胞侵袭能力的影响。方法通过脂质体转染技术,将miR-31转染乳腺癌细胞系过表达miR-31;采用荧光酶报告基因检测miR-31与Dock180的结合方式;应用Western blot法检测miR-31表达不同的乳腺癌细胞中Dock180和其他相关蛋白的表达;RT-PCR检测miR-31与Dock180 mRNA的关系;基质胶侵袭实验检测miR-31过表达时,乳腺癌细胞的侵袭能力。结果在乳腺癌细胞中,miR-31靶向结合Dock180,且Dock180蛋白表达与miR-31表达呈负相关,Dock180下调和miR-31上调削弱了乳腺癌的侵袭能力。结论miR-31可通过结合Dock180抑制乳腺癌细胞的侵袭。
Purpose To investigate the correlation of Dockl80 and miR-31 expression in breast cancer cells, and to observe the effect of miR-31 on the invasion of breast cancer cells by Dock180. Methods MiR-31 was transfected into breast cancer cells by liposome transfection technique. The actual binding site of miR-31 to the 3'-untranslated region of Dock180 was confirmed through luciferase assay. Western blot was performed to detect the expression of Dock180 and other related proteins. Real-time PCR was used to measure the expres-sion of Dock180, Matrigel invasion were performed to detect the invasion of breast cancer cell lines with miR-31 increased. Results The protein levels of Dock180 in breast cancer cell lines negatively correlated with miR-31 expression, and Dockl80 was directly targeted by miR-31. Dock180 downregulation and miR- 31 overexpression reduced breast cancer cells invasion. Conclusion Dock180 modulated by miR-31 plays an important function in breast cancer cell lines invasion.
出处
《临床与实验病理学杂志》
CAS
CSCD
北大核心
2017年第3期241-244,共4页
Chinese Journal of Clinical and Experimental Pathology
基金
国家自然科学基金(81072068
81672631)
山东省自然科学基金中青年科学家奖励基金(2010BSB14051)
山东省教育厅课题(J14LK13)