小麦-近缘物种染色体系耐盐性鉴定及分子标记筛选

Salt Tolerance Identification and Molecular Markers Screening of Wheat-Alien Species Chromosome Lines

为挖掘小麦-近缘物种耐盐种质,对215份小麦-近缘物种染色体系进行了耐盐性筛选与鉴定。结果表明,小麦-高大山羊草6S^l附加系和小麦-纤毛披碱草1Y^c附加系的萌发期耐盐性显著优于普通小麦中国春(CS),但仅后者的幼苗期耐盐性显著优于CS,说明纤毛披碱草1Y^c染色体上可能含有耐盐基因。为了获得1Y^c染色体特异标记,以小麦-纤毛披碱草1Y^c附加系及CS为材料,筛选染色体第一同源群PLUG引物,结果显示,TNAC1007、TNAC1028和TNAC1034为耐盐小麦-纤毛披碱草1Y^c附加系的特异引物,扩增条带大小分别为500bp、700bp和500bp。利用上述3对引物对一套小麦-纤毛披碱草附加系进行扩增,结果发现,上述3个多态性PLUG片段为纤毛披碱草1Y^c和1S^c染色体共有的分子标记,可用于辅助筛选与鉴定小麦-纤毛披碱草1Y^c或1S^c染色体重组体。

In order to explore salt tolerant wheat-alien species germplasm, a total of 215 wheat-alien species chromosome lines were screened and identified. The results showed that the salt tolerance at germination stage of wheat-Aegilops longissirna 6S^c addition line and wheat-Elymus ciliaris 1Y^c addi- tion line are significantly superior to wheat control. However, only the salt tolerance at seedling stage of the latter addition line is significantly superior to wheat control. Therefore,chromosome 1Y^c of E. ciliaris may possess salt tolerant gene. In order to develop molecular markers specific for chromosome 1Y^c, PCR were performed on wheat-E, ciliaris 1Yc addition line and wheat controls by screening group 1 homoeologous chromosome with PLUG primers. The results showed that primers TNAC1007, TNAC1028 and TNAC1034 could generate polymorphic bands at about 500 bp, 700 bp and 500 bp,re- spectively. Subsequently,the above three primer pairs were used to amplify a set of wheat-E, ciliaris additions to locate the polymorphie bands. The result indicates that three polymorphic PLUG seg-ments are not only located on chromosome 1Y^c ,but also on chromosome 1S^c of E. ciliaris. Therefore, these markers can be used in screening and identifying wheat-E, ciliaris chromosome recombinants with 1Y^c or 1S^c involved.